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Comparative Performances of HIV-1 RNA Load Assays at Low Viral Load Levels: Results of an International Collaboration


Swenson, L C; Cobb, B; Geretti, A M; Harrigan, P R; Poljak, M; Seguin-Devaux, C; Verhofstede, Ch; Wirden, M; Amendola, A; Boni, J; Bourlet, T; Huder, J B; Karasi, J B; Zidovec Lepej, S; Lunar, M M; Mukabayire, O; Schuurman, R; Tomazic, J; Van Laethem, K; Vandekerckhove, L; Wensing, A M J (2014). Comparative Performances of HIV-1 RNA Load Assays at Low Viral Load Levels: Results of an International Collaboration. Journal of Clinical Microbiology, 52(2):517-523.

Abstract

Low-level viremia during antiretroviral therapy and its accurate measurement are increasingly relevant. Here, we present an international collaboration of 4,221 paired blood plasma viral load (pVL) results from four commercial assays, emphasizing the data with low pVL. The assays compared were the Abbott RealTime assay, the Roche Amplicor assay, and the Roche TaqMan version 1 and version 2 assays. The correlation between the assays was 0.90 to 0.97. However, at a low pVL, the correlation fell to 0.45 to 0.85. The observed interassay concordance was higher when detectability was defined as 200 copies/ml than when it was defined as 50 copies/ml. A pVL of 100 to 125 copies/ml by the TaqMan version 1 and version 2 assays corresponded best to a 50-copies/ml threshold with the Amplicor assay. Correlation and concordance between the viral load assays were lower at a low pVL. Clear guidelines are needed on the clinical significance of low-level viremia.

Low-level viremia during antiretroviral therapy and its accurate measurement are increasingly relevant. Here, we present an international collaboration of 4,221 paired blood plasma viral load (pVL) results from four commercial assays, emphasizing the data with low pVL. The assays compared were the Abbott RealTime assay, the Roche Amplicor assay, and the Roche TaqMan version 1 and version 2 assays. The correlation between the assays was 0.90 to 0.97. However, at a low pVL, the correlation fell to 0.45 to 0.85. The observed interassay concordance was higher when detectability was defined as 200 copies/ml than when it was defined as 50 copies/ml. A pVL of 100 to 125 copies/ml by the TaqMan version 1 and version 2 assays corresponded best to a 50-copies/ml threshold with the Amplicor assay. Correlation and concordance between the viral load assays were lower at a low pVL. Clear guidelines are needed on the clinical significance of low-level viremia.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Medical Virology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:February 2014
Deposited On:20 Nov 2014 12:26
Last Modified:05 Apr 2016 18:31
Publisher:American Society for Microbiology
ISSN:0095-1137
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1128/JCM.02461-13
PubMed ID:24478482
Permanent URL: https://doi.org/10.5167/uzh-100949

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