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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-10446

Pontiggia, L; Biedermann, T; Meuli, M; Widmer, D; Böttcher-Haberzeth, S; Schiestl, C; Schneider, J; Braziulis, E; Montaño, I; Meuli-Simmen, C; Reichmann, E (2009). Markers to evaluate the quality and self-renewing potential of engineered human skin substitutes in vitro and after transplantation. Journal of Investigative Dermatology, 129(2):480-490.

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Abstract

We screened a series of antibodies for their exclusive binding to the human hair follicle bulge. In a second step these antibodies were to be used to identify basal keratinocytes and potential epithelial stem cells in the human epidermis and in engineered skin substitutes. Of all the antibodies screened, we identified only one, designated C8/144B, that exclusively recognized the hair follicle bulge. However, C8/144B-binding cells were never detected in the human epidermal stratum basale. In the bulge C8/144B-binding cells gave rise to cytokeratin 19-positive cells, which were also tracked in the outer root sheath between bulge and the hair follicle matrix. Remarkably, cytokeratin 19-expressing cells were never detected in the hair follicle infundibulum. Yet, cytokeratin 19-expressing keratinocytes were found in the epidermal stratum basale of normal skin as a subpopulation of cytokeratin 15-positive (not C8/144B-positive) basal keratinocytes. Cytokeratin 19/cytokeratin 15-positive keratinocytes decreased significantly with age. We suggest that cytokeratin 19-expressing cells represent a subpopulation of basal keratinocytes in neonates and young children (up to 1.5 years) that is particularly adapted to the lateral expansion of growing skin. Our data show that cytokeratin 19 in combination with cytokeratin 15 is an important marker to routinely monitor epidermal homeostasis and (at least indirectly) the self-renewing potential of engineered skin.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Children's Hospital Zurich > Clinic for Surgery
DDC:610 Medicine & health
Language:English
Date:21 August 2009
Deposited On:16 Jan 2009 13:14
Last Modified:27 Nov 2013 23:39
Publisher:Nature Publishing Group
ISSN:0022-202X
Additional Information:Originally published in 2009
Publisher DOI:10.1038/jid.2008.254
PubMed ID:18719609
Citations:Web of Science®. Times Cited: 48
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Scopus®. Citation Count: 50

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