Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-1068

Stoeckli, E T; Sonderegger, P; Pollerberg, G E; Landmesser, L T (1997). Interference with axonin-1 and NrCAM interactions unmasks a floor-plate activity inhibitory for commissural axons. Neuron, 18(2):209-221.

[img]
Preview
PDF
1MB

View at publisher

Abstract

Axonin-1 and NrCAM were previously shown to be involved in the in vivo guidance of commissural growth cones across the floor plate of the embryonic chicken spinal cord. To further characterize their role in axon pathfinding, we developed a two-dimensional coculture system of commissural and floor-plate explants in which it was possible to study the behavior of growth cones upon floor-plate contact. Although commissural axons readily entered the floor plate under control conditions, perturbations of either axonin-1 or NrCAM interactions prevented the growth cones from entering the floor-plate explants. The presence of antiaxonin-1 resulted in the collapse of commissural growth cones upon contact with the floor plate. The perturbation of NrCAM interactions also resulted in an avoidance of the floor plate, but without inducing growth-cone collapse. Therefore, axonin-1 and NrCAM are crucial for the contact-mediated interaction between commissural growth cones and the floor plate, which in turn is required for the proper guidance of the axons across the ventral midline and their subsequent rostral turn into the longitudinal axis.

Citations

Altmetrics

Downloads

17 downloads since deposited on 11 Feb 2008
9 downloads since 12 months

Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Department of Biochemistry
07 Faculty of Science > Department of Biochemistry

07 Faculty of Science > Institute of Molecular Life Sciences
DDC:570 Life sciences; biology
Language:English
Date:1 February 1997
Deposited On:11 Feb 2008 12:20
Last Modified:27 Nov 2013 17:31
Publisher:Elsevier
ISSN:0896-6273
Funders:NIH grant NS 19640
Publisher DOI:10.1016/S0896-6273(00)80262-7
Related URLs:http://www.neuron.org/content/article/abstract?uid=PIIS0896627300802627
PubMed ID:9052792

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page