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Berger, P; Kozlov, S V; Cinelli, P; Krüger, S R; Vogt, L; Sonderegger, P (1999). Neuronal depolarization enhances the transcription of the neuronal serine protease inhibitor neuroserpin. Molecular and Cellular Neuroscience, 14(6):455-467.

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Abstract

Neuroserpin is an axonally secreted neuronal serine protease inhibitor. Based on its inhibitory activity towards tissue plasminogen activator (tPA) and its predominant expression in the cerebral cortex, the hippocampus, and the amygdala, a role for neuroserpin in the regulation of neural plasticity has been suggested. We recently found that neuroserpin mRNA is increased in cultured hippocampal neurons upon depolarization with elevated extracellular KCl. Using luciferase reporter constructs containing segments of the promoter region of the neuroserpin gene, we identified a 200-bp segment near the transcription initiation site that is responsible for both the neuron-specific expression of the neuroserpin gene and the enhanced transcription resulting from depolarization. Nerve growth factor, which alone had no effect on the expression of neuroserpin mRNA in hippocampal neurons, had a marked potentiating effect when supplied in combination with elevated extracellular KCl. In contrast, the transcription factor zif/268 blocked neuroserpin transcription. These results implicate neuroserpin as an activity-regulated modulator of tPA activity at the synapse and provide further support for the occurrence of activity-regulated proteolytic processes at the synapse.

Item Type:Journal Article, refereed
Communities & Collections:04 Faculty of Medicine > Institute of Biochemistry
07 Faculty of Science > Institute of Biochemistry
DDC:570 Life sciences; biology
Language:English
Date:01 December 1999
Deposited On:11 Feb 2008 13:20
Last Modified:27 Nov 2013 19:47
Publisher:Elsevier
ISSN:1044-7431
Funders:Swiss National Science Foundation, Helmut Horten Foundation, Olga Mayenfisch Foundation, Jubiläumsstiftung der Rentenanstalt/Swisslife, Novartis Foundation
Publisher DOI:10.1006/mcne.1999.0804
PubMed ID:10656253
Citations:Web of Science®. Times Cited: 35
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