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Effects of L-serine on neurons in vitro


Savoca, R; Ziegler, U; Sonderegger, P (1995). Effects of L-serine on neurons in vitro. Journal of Neuroscience Methods, 61(1-2):159-167.

Abstract

We have examined the influence of amino acids on chicken embryonic dorsal root ganglion (DRG) neurons in vitro and have found that serine has a marked impact on the morphology of the developing neurites. In cultures supplemented with L-serine (but not D-serine) at micromolar concentrations (10-200 microM), the length of the neurites was increased by up to 100% and they were seen to develop a more complex branching pattern. These effects of L-serine were found to be concentration-dependent and stereospecific and were observed on several different substrata such as laminin, Ng-CAM and axonin-1. Similar observations were also made in the case of embryonic retinal explants, while the addition of non-essential amino acids other than L-serine to DRG neurons was found to have no effect. We conclude that, although belonging to the group of non-essential amino acids and not a recognized neurotransmitter, L-serine is an important factor for the morphological differentiation of neurons in vitro.

We have examined the influence of amino acids on chicken embryonic dorsal root ganglion (DRG) neurons in vitro and have found that serine has a marked impact on the morphology of the developing neurites. In cultures supplemented with L-serine (but not D-serine) at micromolar concentrations (10-200 microM), the length of the neurites was increased by up to 100% and they were seen to develop a more complex branching pattern. These effects of L-serine were found to be concentration-dependent and stereospecific and were observed on several different substrata such as laminin, Ng-CAM and axonin-1. Similar observations were also made in the case of embryonic retinal explants, while the addition of non-essential amino acids other than L-serine to DRG neurons was found to have no effect. We conclude that, although belonging to the group of non-essential amino acids and not a recognized neurotransmitter, L-serine is an important factor for the morphological differentiation of neurons in vitro.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Department of Biochemistry
07 Faculty of Science > Department of Biochemistry
Dewey Decimal Classification:570 Life sciences; biology
Language:English
Date:1995
Deposited On:11 Feb 2008 12:20
Last Modified:05 Apr 2016 12:17
Publisher:Elsevier
ISSN:0165-0270
Funders:Swiss National Science Foundation
Publisher DOI:10.1016/0165-0270(95)00038-V
PubMed ID:8618415

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