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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-10890

Haegele, L; Ingold, B; Naumann, H; Tabatabai, G; Ledermann, B; Brandner, S (2003). Wnt signalling inhibits neural differentiation of embryonic stem cells by controlling bone morphogenetic protein expression. Molecular and Cellular Neuroscience, 24(3):696-708.

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Abstract

Wnt signalling plays an important role in both embryonic development and in tumourigenesis. Activation of the signalling cascade by wnt, but also mutations of the adenomatous polyposis coli (APC) protein and of the phosphorylation domain of beta-catenin, result in accumulation of active beta-catenin in the nucleus, where it binds to TCF/LEF transcription factors. We studied the effect of wnt signalling in embryonic stem cells by either inactivating APC or by introducing a dominant active form of beta-catenin. Both resulted in inhibition of neural differentiation in vitro and after brain grafting and in activation of downstream targets of wnt signalling, such as cyclins, c-myc, and bone morphogenetic proteins (BMP). Neural differentiation could be partially restored by the addition of the BMP antagonist noggin. This suggests a mechanism regulating the fate of differentiating embryonic stem cells.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Institute of Neuropathology
05 Vetsuisse Faculty > Institute of Laboratory Animal Science
04 Faculty of Medicine > Institute of Laboratory Animal Science
DDC:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2003
Deposited On:25 Mar 2009 08:04
Last Modified:28 Nov 2013 01:08
Publisher:Elsevier
ISSN:1044-7431
Publisher DOI:10.1016/S1044-7431(03)00232-X
PubMed ID:14664819
Citations:Web of Science®. Times Cited: 68
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Scopus®. Citation Count: 72

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