Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-1122
Fernandez, F J; Vega, M C; Lehmann, F; Sandmeier, E; Gehring, H; Christen, P; Wilmanns, M (2004). Structural studies of the catalytic reaction pathway of a hyperthermophilic histidinol-phosphate aminotransferase. Journal of Biological Chemistry, 279(20):21478-21488.
View at publisher
In histidine biosynthesis, histidinol-phosphate aminotransferase catalyzes the transfer of the amino group from glutamate to imidazole acetol-phosphate producing 2-oxoglutarate and histidinol phosphate. In some organisms such as the hyperthermophile Thermotoga maritima, specific tyrosine and aromatic amino acid transaminases have not been identified to date, suggesting an additional role for histidinol-phosphate aminotransferase in other transamination reactions generating aromatic amino acids. To gain insight into the specific function of this transaminase, we have determined its crystal structure in the absence of any ligand except phosphate, in the presence of covalently bound pyridoxal 5'-phosphate, of the coenzyme histidinol phosphate adduct, and of pyridoxamine 5'-phosphate. The enzyme accepts histidinol phosphate, tyrosine, tryptophan, and phenylalanine, but not histidine, as substrates. The structures provide a model of how these different substrates could be accommodated by histidinol-phosphate aminotransferase. Some of the structural features of the enzyme are more preserved between the T. maritima enzyme and a related threonine-phosphate decarboxylase from S. typhimurium than with histidinol-phosphate aminotransferases from different organisms.
278 downloads since deposited on 11 Feb 2008
92 downloads since 12 months
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > Department of Biochemistry
07 Faculty of Science > Department of Biochemistry
|Dewey Decimal Classification:||570 Life sciences; biology|
|Date:||14 May 2004|
|Deposited On:||11 Feb 2008 12:20|
|Last Modified:||27 Nov 2013 22:43|
|Publisher:||American Society for Biochemistry and Molecular Biology|
Users (please log in): suggest update or correction for this item
Repository Staff Only: item control page