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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-11304

Osto, E; Matter, C M; Kouroedov, A; Malinski, T; Bachschmid, M; Camici, G G; Kilic, U; Stallmach, T; Boren, J; Iliceto, S; Lüscher, T F; Cosentino, F (2008). c-Jun N-terminal kinase 2 deficiency protects against hypercholesterolemia-induced endothelial dysfunction and oxidative stress. Circulation, 118(20):2073-2080.

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Abstract

BACKGROUND: Hypercholesterolemia-induced endothelial dysfunction due to excessive production of reactive oxygen species is a major trigger of atherogenesis. The c-Jun-N-terminal kinases (JNKs) are activated by oxidative stress and play a key role in atherogenesis and inflammation. We investigated whether JNK2 deletion protects from hypercholesterolemia-induced endothelial dysfunction and oxidative stress. METHODS AND RESULTS: Male JNK2 knockout (JNK2(-/-)) and wild-type (WT) mice (8 weeks old) were fed either a high-cholesterol diet (HCD; 1.25% total cholesterol) or a normal diet for 14 weeks. Aortic lysates of WT mice fed a HCD showed an increase in JNK phosphorylation compared with WT mice fed a normal diet (P<0.05). Endothelium-dependent relaxations to acetylcholine were impaired in WT HCD mice (P<0.05 versus WT normal diet). In contrast, JNK2(-/-) HCD mice did not exhibit endothelial dysfunction (96+/-5% maximal relaxation in response to acetylcholine; P<0.05 versus WT HCD). Endothelium-independent relaxations were identical in all groups. A hypercholesterolemia-induced decrease in nitric oxide (NO) release of endothelial cells was found in WT but not in JNK2(-/-) mice. In parallel, endothelial NO synthase expression was upregulated only in JNK2(-/-) HCD animals, whereas the expression of antioxidant defense systems such as extracellular superoxide dismutase and manganese superoxide dismutase was decreased in WT but not in JNK2(-/-) HCD mice. In contrast to JNK2(-/-) mice, WT HCD displayed an increase in O(2)(-) and ONOO(-) concentrations as well as nitrotyrosine staining and peroxidation. CONCLUSIONS: JNK2 plays a critical role as a mediator of hypercholesterolemia-induced endothelial dysfunction and oxidative stress. Thus, JNK2 may provide a novel target for prevention of vascular disease and atherosclerosis.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Center for Integrative Human Physiology
04 Faculty of Medicine > University Hospital Zurich > Institute of Surgical Pathology
04 Faculty of Medicine > University Hospital Zurich > Clinic for Cardiology
DDC:570 Life sciences; biology
610 Medicine & health
Language:English
Date:November 2008
Deposited On:23 Jan 2009 10:36
Last Modified:07 Dec 2013 16:44
Publisher:Lippincott Wiliams & Wilkins
ISSN:0009-7322
Funders:Swiss National Research Foundation grants, Swiss Heart Foundation, US Public Health grant, URPP "Integrative Human Physiology", European Vascular Genomic Network, Mercator Foundation
Additional Information:This is a non-final version of an article published in final form in Circulation
Publisher DOI:10.1161/CIRCULATIONAHA.108.765032
PubMed ID:18955669
Citations:Web of Science®. Times Cited: 33
Google Scholar™
Scopus®. Citation Count: 33

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