Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-11348

Zimmermann, J; Rabe, M; Verdes, D; Seeger, S (2008). Functionalized silicone nanofilaments: a novel material for selective protein enrichment. Langmuir, 24(3):1053-1057.

[img] PDF - Registered users only
1MB

View at publisher

Abstract

We present a simple and versatile technique of tailoring functionalized surface structures for protein enrichment
and purification applications based on a superhydrophobic silicone nanofilament coating. Using amino and carboxyl
group containing silanes, silicone nanofilament templates were chemically modified to mimic anionic and cationic
exchange resins. Investigations on the selectivity of the functionalized surfaces toward adsorption of charged model
proteins were carried out by means of fluorescence techniques. Due to a high contact area resulting from the nanoroughness
of the coating, excellent protein retention characteristics under various conditions were found. The surfaces were
shown to be highly stable and reusable over several retention-elution cycles. Especially the full optical transparency
and the possibility to use glass substrates as support material open new opportunities for the development of optical
biosensors, open geometry microfluidics, or lab-on-a-chip devices.

Citations

25 citations in Web of Science®
25 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

0 downloads since deposited on 25 Jan 2009
0 downloads since 12 months

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Chemistry
DDC:540 Chemistry
Language:English
Date:February 2008
Deposited On:25 Jan 2009 16:53
Last Modified:05 Jun 2014 13:51
Publisher:American Chemical Society
ISSN:0743-7463
Publisher DOI:10.1021/la702977v
PubMed ID:18154313

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page