Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-11534
Ries, J; Ruckstuhl, T; Verdes, D; Schwille, P (2008). Supercritical angle fluorescence correlation spectroscopy. Biophysical Journal, 94(1):221-229.
View at publisher
We explore the potential of a supercritical angle (SA) objective for fluorescence correlation spectroscopy (FCS). This novel microscope objective combines tight focusing by an aspheric lens with strong axial confinement of supercritical angle fluorescence collection by a parabolic mirror lens, resulting in a small detection volume. The tiny axial extent of the detection volume features an excellent surface sensitivity, as is demonstrated by diffusion measurements in model membranes with an excess of free dye in solution. All SA-FCS measurements are directly compared to standard confocal FCS, demonstrating a clear advantage of SA-FCS, especially for diffusion measurements in membranes. We present an extensive theoretical framework that allows for accurate and quantitative evaluation of the SA-FCS correlation curves.
147 downloads since deposited on 24 Jan 2009
27 downloads since 12 months
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||07 Faculty of Science > Department of Chemistry|
|Dewey Decimal Classification:||540 Chemistry|
|Deposited On:||24 Jan 2009 16:48|
|Last Modified:||05 Jun 2014 13:51|
Users (please log in): suggest update or correction for this item
Repository Staff Only: item control page