Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-11534
Ries, J; Ruckstuhl, T; Verdes, D; Schwille, P (2008). Supercritical angle fluorescence correlation spectroscopy. Biophysical Journal, 94(1):221-229.
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We explore the potential of a supercritical angle (SA) objective for fluorescence correlation spectroscopy (FCS). This novel microscope objective combines tight focusing by an aspheric lens with strong axial confinement of supercritical angle fluorescence collection by a parabolic mirror lens, resulting in a small detection volume. The tiny axial extent of the detection volume features an excellent surface sensitivity, as is demonstrated by diffusion measurements in model membranes with an excess of free dye in solution. All SA-FCS measurements are directly compared to standard confocal FCS, demonstrating a clear advantage of SA-FCS, especially for diffusion measurements in membranes. We present an extensive theoretical framework that allows for accurate and quantitative evaluation of the SA-FCS correlation curves.
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|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||07 Faculty of Science > Department of Chemistry|
|Deposited On:||24 Jan 2009 16:48|
|Last Modified:||05 Jun 2014 13:51|
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