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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-11976

Zini, E; Linscheid, P; Franchini, M; Kaufmann, K; Monnais, E; Kutter, A P N; Ackermann, M; Lutz, T A; Reusch, C R (2009). Partial sequencing and expression of genes involved in glucose metabolism in adipose tissues and skeletal muscle of healthy cats. Veterinary Journal, 180(1):66-70.

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Impaired insulin sensitivity is increasingly recognised in cats, but sequences of genes involved in insulin-signalling are largely undetermined in this species. In this study, extended feline mRNA sequences were determined for the adiponectin, glucose transporter-1 (GLUT1), GLUT4, peroxisome proliferative activated receptor-gamma1 (PPARgamma1), PPARgamma2, plasminogen activator inhibitor-1 (PAI-1), monocyte chemoattractant protein-1 (MCP-1) and insulin receptor genes. Conserved dog-specific primers identified from human-dog mRNA alignments were used to amplify feline cDNA in the polymerase chain reaction (PCR). The feline sequences determined by this method were used to design feline-specific primers suitable for real-time PCR for quantification of gene expression in insulin sensitive tissues of healthy cats. Partial sequences of feline mRNAs had 86-95% identity with dog and human genes. Expression of adiponectin, GLUT1, GLUT4, PPARgamma1, PPARgamma2, PAI-1 and insulin receptor mRNA was detected and quantified in subcutaneous and visceral fat and skeletal muscle, whereas MCP-1 mRNA was detected in adipose tissue but not in skeletal muscle. Further characterisation of genes related to glucose metabolism in cats will provide additional insights into insulin-signalling mechanisms in this species.

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Veterinary Physiology
05 Vetsuisse Faculty > Veterinary Clinic > Department of Small Animals > Clinic for Small Animal Internal Medicine
05 Vetsuisse Faculty > Veterinary Clinic > Equine Department > Equine Clinic
05 Vetsuisse Faculty > Institute of Virology
DDC:570 Life sciences; biology
630 Agriculture
Date:April 2009
Deposited On:28 Jan 2009 15:16
Last Modified:27 Nov 2013 22:34
Publisher DOI:10.1016/j.tvjl.2007.10.022
PubMed ID:18078768
Citations:Web of Science®. Times Cited: 11
Google Scholar™
Scopus®. Citation Count: 14

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