UZH-Logo

Maintenance Infos

LPS-mediated effects and spatio-temporal expression of TLR2 and TLR4 in the bovine corpus luteum


Lüttgenau, Johannes; Herzog, Kathrin; Strüve, Klaas; Latter, Sophie; Boos, Alois; Bruckmaier, Rupert M; Bollwein, Heinrich; Kowalewski, Mariusz Pawel (2016). LPS-mediated effects and spatio-temporal expression of TLR2 and TLR4 in the bovine corpus luteum. Reproduction, 151:391-399.

Abstract

When given intravenously (iv), lipopolysaccharide (LPS) transiently suppresses the structure and function of the bovine corpus luteum (CL). This is associated with increased release of prostaglandin (PG) F2α metabolite (PGFM). The underlying regulatory mechanisms of this process remain, however, obscure. Therefore, the aims of this study were: (1) to investigate the expression of the LPS receptor toll-like receptor (TLR) 4 and TLR2 in the bovine CL during early, mid, and late luteal phases; (2) to further dissect the mechanisms of LPS-mediated suppression of luteal function. As revealed by semi-quantitative qPCR and immunohistochemistry, both receptors were detectable throughout the luteal lifespan. Their mRNA levels increased from the early towards the mid-luteal phase; no further changes were observed thereafter. The TLR4 protein seemed more highly represented than TLR2. The cellular localization of TLRs was in blood vessels; weaker signals were observed in luteal cells. Additionally, cows were treated either with LPS (iv, 0.5 µg/kgBW) or with saline on Day 10 after ovulation. Samples were collected 12 h after treatment, and on Day 10 of the respective subsequent (untreated) cycle. The mRNA expression of several possible regulatory factors was investigated, revealing the suppression of PGF2α receptor (PTGFR), steroidogenic acute regulatory protein (STAR) and 3β-hydroxysteroid dehydrogenase (3βHSD), compared with controls and subsequent cycles. The expression of TLR2 and TLR4, interleukin (IL) 1α and IL1β, and of PGF2α and PGE2 synthases (20αHSD/PGFS and mPTGES, respectively) was increased. The results demonstrate the presence of TLR2 and TLR4 in the bovine CL, and implicate their possible involvement in the deleterious effects of LPS on its function.

Abstract

When given intravenously (iv), lipopolysaccharide (LPS) transiently suppresses the structure and function of the bovine corpus luteum (CL). This is associated with increased release of prostaglandin (PG) F2α metabolite (PGFM). The underlying regulatory mechanisms of this process remain, however, obscure. Therefore, the aims of this study were: (1) to investigate the expression of the LPS receptor toll-like receptor (TLR) 4 and TLR2 in the bovine CL during early, mid, and late luteal phases; (2) to further dissect the mechanisms of LPS-mediated suppression of luteal function. As revealed by semi-quantitative qPCR and immunohistochemistry, both receptors were detectable throughout the luteal lifespan. Their mRNA levels increased from the early towards the mid-luteal phase; no further changes were observed thereafter. The TLR4 protein seemed more highly represented than TLR2. The cellular localization of TLRs was in blood vessels; weaker signals were observed in luteal cells. Additionally, cows were treated either with LPS (iv, 0.5 µg/kgBW) or with saline on Day 10 after ovulation. Samples were collected 12 h after treatment, and on Day 10 of the respective subsequent (untreated) cycle. The mRNA expression of several possible regulatory factors was investigated, revealing the suppression of PGF2α receptor (PTGFR), steroidogenic acute regulatory protein (STAR) and 3β-hydroxysteroid dehydrogenase (3βHSD), compared with controls and subsequent cycles. The expression of TLR2 and TLR4, interleukin (IL) 1α and IL1β, and of PGF2α and PGE2 synthases (20αHSD/PGFS and mPTGES, respectively) was increased. The results demonstrate the presence of TLR2 and TLR4 in the bovine CL, and implicate their possible involvement in the deleterious effects of LPS on its function.

Citations

2 citations in Web of Science®
2 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

1 download since deposited on 18 Feb 2016
1 download since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Veterinary Anatomy
05 Vetsuisse Faculty > Institute of Veterinary Physiology
05 Vetsuisse Faculty > Veterinary Clinic > Department of Farm Animals
Dewey Decimal Classification:570 Life sciences; biology
630 Agriculture
Language:English
Date:13 January 2016
Deposited On:18 Feb 2016 10:59
Last Modified:05 Apr 2016 20:02
Publisher:BioScientifica Ltd.
ISSN:1470-1626
Additional Information:This is not the definitive version of record of this article. This manuscript has been accepted for publication in Reproduction, but the version presented here has not yet been copy-edited, formatted or proofed. Consequently, Bioscientifica accepts no responsibility for any errors or omissions it may contain. The definitive version is now freely available at http://doi.org/10.1530/REP-15-0520 2016.
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1530/REP-15-0520
PubMed ID:26762400

Download

[img]
Content: Accepted Version
Filetype: PDF - Registered users only until 14 January 2017
Size: 782kB
View at publisher
Embargo till: 2017-01-14

TrendTerms

TrendTerms displays relevant terms of the abstract of this publication and related documents on a map. The terms and their relations were extracted from ZORA using word statistics. Their timelines are taken from ZORA as well. The bubble size of a term is proportional to the number of documents where the term occurs. Red, orange, yellow and green colors are used for terms that occur in the current document; red indicates high interlinkedness of a term with other terms, orange, yellow and green decreasing interlinkedness. Blue is used for terms that have a relation with the terms in this document, but occur in other documents.
You can navigate and zoom the map. Mouse-hovering a term displays its timeline, clicking it yields the associated documents.

Author Collaborations