UZH-Logo

Maintenance Infos

Polar body diagnosis (PBD): an alternative and supplement to preimplantation diagnosis for single embryo transfer


Imthurn, B; Berger, W; Macas, E; Magyar, I; Oneda, Beatrice; Rauch, A; Xie, M (2015). Polar body diagnosis (PBD): an alternative and supplement to preimplantation diagnosis for single embryo transfer. In: Sills, E Scott. Screening the Single Euploid Embryo. International Publishing Switzerland: Springer, 103-122.

Abstract

Polar body diagnosis (PBD) is an accurate but also costly method of genetic preconception diagnosis. It is used mainly in countries with legal restrictions against preimplantation genetic diagnosis (PGD) of cleaved embryos. The lack of mosaicism is its predominant advantage; the limitation to genetic problems of maternal origin is its principal disadvantage. The first polar body (PB1) is removed before fertilization and the second polar body (PB2) (or both PBs together) at the time of fertilization. Mechanical opening of the zona pellucida is the recommended means of obtaining access to the PBs. There are several techniques available for the genetic analysis of PBs, but the most commonly used today are array-CGH and PCR. After biopsy of the PBs, vitrification of pronuclear stages or cleaved embryos/blastocysts and the subsequent transfer of unaffected embryos/blastocysts in artificial warming cycles allow the time window for genetic testing to be expanded. Misdiagnosis in PBD is known anecdotally. The future of PBD is dependent on both the legal situation and the results of urgently needed prospectively randomized studies including both PBD and PID of cleaved embryos and blastocysts. The combination of PBD with trophectoderm PGD might increase the accuracy of both techniques, particularly when the aim is to transfer only one embryo.

Abstract

Polar body diagnosis (PBD) is an accurate but also costly method of genetic preconception diagnosis. It is used mainly in countries with legal restrictions against preimplantation genetic diagnosis (PGD) of cleaved embryos. The lack of mosaicism is its predominant advantage; the limitation to genetic problems of maternal origin is its principal disadvantage. The first polar body (PB1) is removed before fertilization and the second polar body (PB2) (or both PBs together) at the time of fertilization. Mechanical opening of the zona pellucida is the recommended means of obtaining access to the PBs. There are several techniques available for the genetic analysis of PBs, but the most commonly used today are array-CGH and PCR. After biopsy of the PBs, vitrification of pronuclear stages or cleaved embryos/blastocysts and the subsequent transfer of unaffected embryos/blastocysts in artificial warming cycles allow the time window for genetic testing to be expanded. Misdiagnosis in PBD is known anecdotally. The future of PBD is dependent on both the legal situation and the results of urgently needed prospectively randomized studies including both PBD and PID of cleaved embryos and blastocysts. The combination of PBD with trophectoderm PGD might increase the accuracy of both techniques, particularly when the aim is to transfer only one embryo.

Altmetrics

Additional indexing

Item Type:Book Section, refereed, further contribution
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Clinic for Reproductive Endocrinology
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:2015
Deposited On:01 Feb 2016 16:10
Last Modified:05 Apr 2016 20:03
Publisher:Springer
ISBN:978-3-319-16892-0
Publisher DOI:https://doi.org/10.1007/978-3-319-16892-0_8

Download

Full text not available from this repository.
View at publisher

TrendTerms

TrendTerms displays relevant terms of the abstract of this publication and related documents on a map. The terms and their relations were extracted from ZORA using word statistics. Their timelines are taken from ZORA as well. The bubble size of a term is proportional to the number of documents where the term occurs. Red, orange, yellow and green colors are used for terms that occur in the current document; red indicates high interlinkedness of a term with other terms, orange, yellow and green decreasing interlinkedness. Blue is used for terms that have a relation with the terms in this document, but occur in other documents.
You can navigate and zoom the map. Mouse-hovering a term displays its timeline, clicking it yields the associated documents.

Author Collaborations