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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-122

Litwack, E D; Babey, R; Buser, R; Gesemann, M; O'Leary, D D M (2004). Identification and characterization of two novel brain-derived immunoglobulin superfamily members with a unique structural organization. Molecular and Cellular Neuroscience, 25(2):263-274.

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Abstract

We recently used a differential display PCR screen to identify secreted and transmembrane proteins that are highly expressed in the developing rat basilar pons, a prominent ventral hindbrain nucleus used as a model for studies of neuronal migration, axon outgrowth, and axon-target recognition. Here we describe cloning and characterization of one of these molecules, now called MDGA1, and a closely related homologue, MDGA2. Analyses of the full-length coding region of MDGA1 and MDGA2 indicate that they encode proteins that comprise a novel subgroup of the Ig superfamily and have a unique structural organization consisting of six immunoglobulin (Ig)-like domains followed by a single MAM domain. Biochemical characterization demonstrates that MDGA1 and MDGA2 proteins are highly glycosylated, and that MDGA1 is tethered to the cell membrane by a GPI anchor. The MDGAs are differentially expressed by subpopulations of neurons in both the central and peripheral nervous systems, including neurons of the basilar pons, inferior olive, cerebellum, cerebral cortex, olfactory bulb, spinal cord, and dorsal root and trigeminal ganglia. Little or no MDGA expression is detected outside of the nervous system of developing rats. The similarity of MDGAs to other Ig-containing molecules and their temporal-spatial patterns of expression within restricted neuronal populations, for example migrating pontine neurons and D1 spinal interneurons, suggest a role for these novel proteins in regulating neuronal migration, as well as other aspects of neural development, including axon guidance.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Brain Research Institute
DDC:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2004
Deposited On:11 Feb 2008 13:12
Last Modified:04 Dec 2013 02:36
Publisher:Elsevier
ISSN:1044-7431
Publisher DOI:10.1016/j.mcn.2003.10.016
PubMed ID:15019943
Citations:Web of Science®. Times Cited: 27
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