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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-12514

Wyss, M T; Weber, B; Treyer, V; Heer, S; Pellerin, L; Magistretti, P J; Buck, A (2009). Stimulation-induced increases of astrocytic oxidative metabolism in rats and humans investigated with 1-11C-acetate. Journal of Cerebral Blood Flow and Metabolism, 29(1):44-56.

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Abstract

The purpose of this study was to investigate astrocytic oxidative metabolism using 1-(11)C-acetate. 1-(11)C-acetate kinetics were evaluated in the rat somatosensory cortex using a beta-scintillator during different manipulations (test-retest, infraorbital nerve stimulation, and administration of acetazolamide or dichloroacetate). In humans a visual activation paradigm was used and kinetics were measured with positron emission tomography. Data were analyzed using a one-tissue compartment model. The following features supported the hypothesis that washout of radiolabel (k(2)) is because of (11)C-CO(2) and therefore related to oxygen consumption (CMRO(2)): (1) the onset of (11)C washout was delayed; (2)k(2) was not affected by acetazolamide-induced blood flow increase; (3)k(2) demonstrated a significant increase during stimulation in rats (from 0.014+/-0.007 to 0.027+/-0.006 per minute) and humans (from 0.016+/-0.010 to 0.026+/-0.006 per minute); and (4) dichloroacetate led to a substantial decrease of k(2). In the test-retest experiments K(1) and k(2) were very stable. In summary, 1-(11)C-acetate seems a promising tracer to investigate astrocytic oxidative metabolism in vivo. If the washout rate indeed represents the production of (11)C-CO(2), then its increase during stimulation would point to a substantially higher astrocytic oxidative metabolism during brain activation. However, the quantitative relationship between k(2) and CMRO(2) needs to be determined in future experiments.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Clinic for Nuclear Medicine
04 Faculty of Medicine > Institute of Pharmacology and Toxicology
DDC:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2009
Deposited On:12 Mar 2009 12:32
Last Modified:27 Nov 2013 22:13
Publisher:Nature Publishing Group
ISSN:0271-678X
Additional Information:Nature Publishing Group - Full text article
Publisher DOI:10.1038/jcbfm.2008.86
Official URL:http://www.nature.com/jcbfm/journal/v29/n1/pdf/jcbfm200886a.pdf
PubMed ID:18714330
Citations:Web of Science®. Times Cited: 18
Google Scholar™
Scopus®. Citation Count: 19

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