Eckhardt, K; Troger, J; Reissmann, J; Katschinski, D M; Wagner, K F; Stengel, P J; Paasch, U; Hunziker, P; Borter, E; Barth, S; Schlafli, P; Spielmann, P; Stiehl, D P; Camenisch, G; Wenger, R H (2007). Male germ cell expression of the PAS domain kinase PASKIN and its novel target eukaryotic translation elongation factor eEF1A1. Cellular Physiology and Biochemistry, 20(1-4):227-240.
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PASKIN links energy flux and protein synthesis in yeast, regulates glycogen synthesis in mammals, and has been implicated in glucose-stimulated insulin production in pancreatic beta-cells. Using newly generated monoclonal antibodies, PASKIN was localized in the nuclei of human testis germ cells and in the midpiece of human sperm tails. A speckle-like nuclear pattern was observed for endogenous PASKIN in HeLa cells in addition to its cytoplasmic localization. By yeast two-hybrid screening, we identified the multifunctional eukaryotic translation elongation factor eEF1A1 as a novel interaction partner of PASKIN. This interaction was mapped to the PAS A and kinase domains of PASKIN and to the C-terminus of eEF1A1 using mammalian two-hybrid and GST pull-down assays. Kinase assays, mass spectrometry and site-directed mutagenesis revealed PASKIN auto-phosphorylation as well as eEF1A1 target phosphorylation mainly but not exclusively at Thr432. Wild-type but not kinase-inactive PASKIN increased the in vitro translation of a reporter cRNA. Whereas eEF1A1 did not localize to the nucleus, it co-localizes with PASKIN to the cytoplasm of HeLa cells. The two proteins also showed a remarkably similar localization in the midpiece of the sperm tail. These data suggest regulation of eEF1A1 by PASKIN-dependent phosphorylation in somatic as well as in sperm cells.
|Item Type:||Journal Article, refereed|
|Communities & Collections:||04 Faculty of Medicine > Functional Genomics Center Zurich
04 Faculty of Medicine > Institute of Physiology
07 Faculty of Science > Institute of Physiology
08 University Research Priority Programs > Systems Biology / Functional Genomics
|DDC:||570 Life sciences; biology
610 Medicine & health
|Deposited On:||11 Feb 2008 12:21|
|Last Modified:||27 Nov 2013 18:31|
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