Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-12933
Altintas, M A; Altintas, A A; Guggenheim, M; Gohritz, A; Meyer-Marcotty, M; Vogt, P M (2009). Reflectance confocal-laser-scanning microscopy in vivo assessments of cigarette-induced dynamic alterations of cutaneous microcirculation on histomorphological level. Microscopy Research and Technique, 72(4):347-350.
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Objective: Until now, high resolution reflectance confocal-laser-scanning microscopy (CLSM) was used for observation of cutaneous morphology in vivo and in real time. We hypothesized that CLSM also allows observation of dynamic processes of cutaneous microcirculation. Methods: Reflectance CLSM (Vivascope1500; Lucid, Rochester, NY) was performed in 24 young male habitual smokers (23 years, range: 19-26, body mass index 23.9 +/- 4.04) with relatively limited cigarette exposure (mean: 3.1 +/- 2.4 pack-years). Eight matched nonsmokers served as controls. The quantitative blood cell flow and the diameter of capillary loops were determined prior (baseline), during, as well as 5 and 10 min after smoking. Results: Baseline value for blood cell flow was 55.50 +/- 2.33 cells/min, and decreased over 45% during smoking (30.43 +/- 3.76/min; P = 0.02). They were still 22% lower (43.33 +/- 2.45/min; P = 0.01) 5 min after smoking and exceeded baseline values 10 min after smoking by 13% (63.00 +/- 3.10/min; P > 0.05). The baseline values for capillary loop diameter (9.03 +/- 0.22 mum) decreased by 21% (7.18 +/- 0.28 mum; P = 0.03) during smoking, remained about 9% (8.23 +/- 0.18 mum; P = 0.01) lower 5 min after smoking and exceeded baseline values insignificantly by 4% (9.38 +/- 0.28 mum; P > 0.05) 10 min after smoking. There were no significant differences to the controls. Conclusion: Reflectance CLSM enables qualitative and quantitative observation of dynamic processes of cutaneous microcirculation on histomorphological level. Microsc. Res. Tech., 2009. (c) 2008 Wiley-Liss, Inc.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > University Hospital Zurich > Clinic for Reconstructive Surgery|
04 Faculty of Medicine > University Hospital Zurich > Division of Surgical Research
|DDC:||610 Medicine & health|
|Date:||09 April 2009|
|Deposited On:||13 Feb 2009 15:42|
|Last Modified:||03 Dec 2012 22:08|
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