Sorribas, V; Markovich, D; Verri, T; Biber, J; Murer, H (1995). Thyroid hormone stimulation of Na/Pi-cotransport in opossum kidney cells. Pflügers Archiv: European Journal of Physiology (Pflugers Archiv), 431(2):266-271.
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Thyroid hormone (T3), a known stimulator of renal proximal tubular brush border membrane Na-dependent phosphate (Pi) uptake (Na/Pi-cotransport), stimulated Na-dependent Pi transport in opossum kidney (OK) cells. Na/Pi-cotransport was stimulated in a time- and dose-dependent manner with maximal effects (57%) at 24 h and 10(-10) M T3. This stimulation was related to an increase in the apparent capacity (Vmax) of Na/Pi-cotransport. Treatment with T3 had no effect on Na-independent transport of Pi or of L-arginine. The stimulation of Na/Pi-cotransport was paralleled by an increase in the messenger ribonucleic acid (mRNA) encoding the OK cell apical Na/Pi-cotransporter (termed NaPi-4); the mRNA levels related to the activity of Na-independent L-arginine transport (rBAT) were unaffected by T3. Actinomycin D (10(-7) M) completely prevented the stimulatory effect of T3 on OK cell Na/Pi-cotransport and on NaPi-4 mRNA content. In conclusion, T3 stimulates apical Na/Pi-cotransport in OK cells most likely by enhancing its transcription.
|Item Type:||Journal Article, refereed|
|Communities & Collections:||04 Faculty of Medicine > Institute of Physiology
07 Faculty of Science > Institute of Physiology
|Dewey Decimal Classification:||570 Life sciences; biology|
|Date:||1 December 1995|
|Deposited On:||11 Feb 2008 12:22|
|Last Modified:||05 Apr 2016 12:18|
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