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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-1309

Gisler, S M; Stagljar, I; Traebert, M; Bacic, D; Biber, J; Murer, H (2001). Interaction of the type IIa Na/Pi cotransporter with PDZ proteins. Journal of Biological Chemistry, 276(12):9206-9213.

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The type IIa Na(+)-dependent inorganic phosphate (Na/P(i)) cotransporter is localized in the apical membrane of proximal tubular cells and is regulated by an endocytotic pathway. Because molecular processes such as apical sorting, internalization, or subsequent degradation might be assisted by associated proteins, a yeast two-hybrid screen against the C-terminal, cytosolic tail of type IIa cotransporter was designed. Most of the potential proteins found belonged to proteins with multiple PDZ modules and were either identical/related to PDZK1 or identical to NHERF-1. Yeast trap truncation assays confined the peptide-protein association to the C-terminal amino acid residues TRL of type IIa cotransporter and to single PDZ domains of each identified protein, respectively. The specificity of these interactions were confirmed in yeast by testing other apical localized transmembraneous proteins. Moreover, the type IIa protein was recovered in vitro by glutathione S-transferase-fused PDZ proteins from isolated renal brush border membranes or from type IIa-expressing oocytes. Further, these PDZ proteins are immunohistochemically detected either in the microvilli or in the subapical compartment of proximal tubular cells. Our results suggest that the type IIa Na/P(i) cotransporter interacts with various PDZ proteins that might be responsible for the apical sorting, parathyroid hormone controlled endocytosis or the lysosomal sorting of internalized type IIa cotransporter.


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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Physiology
07 Faculty of Science > Institute of Physiology
Dewey Decimal Classification:570 Life sciences; biology
Date:23 March 2001
Deposited On:11 Feb 2008 12:22
Last Modified:05 Apr 2016 12:18
Publisher:American Society for Biochemistry and Molecular Biology
Additional Information:This research was originally published in Gisler, S M; Stagljar, I; Traebert, M; Bacic, D; Biber, J; Murer, H. Interaction of the type IIa Na/Pi cotransporter with PDZ proteins. J. Biol. Chem. 2001, 276(12):9206-13. © the American Society for Biochemistry and Molecular Biology.
Publisher DOI:10.1074/jbc.M008745200
PubMed ID:11099500

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