Hernando, N; Forgo, J; Biber, J; Murer, H (2000). PTH-Induced downregulation of the type IIa Na/P(i)-cotransporter is independent of known endocytic motifs. Journal of the American Society of Nephrology (JASN), 11(11):1961-1968.
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Parathyroid hormone (PTH)-induced inhibition of renal proximal tubular Na/P(i) cotransport involves two consecutive steps: endocytosis followed by lysosomal degradation of the type IIa Na/P(i) cotransporter. Tyrosine-, dileucine-, and diacidic-based motifs are suggested to be involved in endocytosis and/or lysosomal targeting of different plasma membrane proteins. The rat type IIa cotransporter (NaPi2) contains two cytoplasmic tyrosine residues (Y) within sequences highly homologous to tyrosine-based motifs (GY(402)FAM and Y(509)RWF), three cytoplasmic dileucine (LL(101), LL(374), and LI(591)) and two cytoplasmic diacidic motifs (EE(81) and EE(616)). We studied the role of these motifs on the PTH-induced retrieval and lysosomal degradation of the NaPi2 cotransporter. To follow its trafficking in vivo, the NaPi2 protein was fused to the carboxyl-terminal end of the enhanced green fluorescence protein. This fusion did not impair the apical targeting or the PTH-induced endocytosis of the wild-type cotransporter when transfected in opossum kidney cells. Single and multiple Y and LL mutants retained the apical targeting and the PTH-induced degradation. Mutations of the diacidic motifs were also without effect. These data suggest that the above three motifs are not required for the PTH-induced internalization and/or degradation of the cotransporter.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > Institute of Physiology|
07 Faculty of Science > Institute of Physiology
|DDC:||570 Life sciences; biology|
|Date:||01 November 2000|
|Deposited On:||11 Feb 2008 13:22|
|Last Modified:||27 Nov 2013 21:11|
|Publisher:||American Society of Nephrology|
|Citations:||Web of Science®. Times cited: 25|
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