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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-13401

Lehmann, R; Zuellig, R A; Kugelmeier, P; Baenninger, P B; Moritz, W; Perren, A; Clavien, P A; Weber, M; Spinas, G A (2007). Superiority of small islets in human islet transplantation. Diabetes, 56(3):594-603.

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Abstract

Many factors influence the outcome of islet transplantation. As islets in the early posttransplant setting are supplied with oxygen by diffusion only and are in a hypoxic state in the portal system, we tested whether small human islets are superior to large islets both in vitro and in vivo. We assessed insulin secretion of large and small islets and quantified cell death during hypoxic conditions simulating the intraportal transplant environment. In the clinical setting, we analyzed the influence of transplanted islet size on insulin production in patients with type 1 diabetes. Our results provide evidence that small islets are superior to large islets with regard to in vitro insulin secretion and show a higher survival rate during both normoxic and hypoxic culture. Islet volume after 48 h of hypoxic culture decreased to 25% compared with normoxic culture at 24 h due to a preferential loss of large islets. In human islet transplantation, the isolation index (islet volume as expressed in islet equivalents/islet number), or more simply the islet number, proved to be more reliable to predict stimulated C-peptide response compared with islet volume. Thus, islet size seems to be a key factor determining human islet transplantation outcome.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Clinic for Endocrinology and Diabetology
05 Vetsuisse Faculty > Institute of Veterinary Physiology
04 Faculty of Medicine > Center for Integrative Human Physiology
04 Faculty of Medicine > University Hospital Zurich > Clinic for Visceral and Transplantation Surgery
DDC:570 Life sciences; biology
610 Medicine & health
Language:English
Date:March 2007
Deposited On:18 Mar 2009 12:22
Last Modified:28 Nov 2013 00:45
Publisher:American Diabetes Association
ISSN:0012-1797
Publisher DOI:10.2337/db06-0779
PubMed ID:17327426
Citations:Web of Science®. Times Cited: 95
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