Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive 

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-13901

Hoek, K S; Schlegel, N C; Eichhoff, O M; Widmer, D S; Praetorius, C; Einarsson, S O; Valgeirsdottir, S; Bergsteinsdottir, K; Schepsky, A; Dummer, R; Steingrimsson, E (2008). Novel MITF targets identified using a two-step DNA microarray strategy. Pigment Cell & Melanoma Research, 21(6):665-676.

[img]PDF - Registered users only
2121Kb

Abstract

Malignant melanoma is a chemotherapy-resistant cancer with high mortality. Recent advances in our understanding of the disease at the molecular level have indicated that it shares many characteristics with developmental precursors to melanocytes, the mature pigment-producing cells of the skin and hair follicles. The development of melanocytes absolutely depends on the action of the microphthalmia-associated transcription factor (MITF). MITF has been shown to regulate a broad variety of genes, whose functions range from pigment production to cell-cycle regulation, migration and survival. However, the existing list of targets is not sufficient to explain the role of MITF in melanocyte development and melanoma progression. DNA microarray analysis of gene expression offers a straightforward approach to identify new target genes, but standard analytical procedures are susceptible to the generation of false positives and require additional experimental steps for validation. Here, we introduce a new strategy where two DNA microarray-based approaches for identifying transcription factor targets are combined in a cross-validation protocol designed to help control false-positive generation. We use this two-step approach to successfully re-identify thirteen previously recorded targets of MITF-mediated upregulation, as well as 71 novel targets. Many of these new targets have known relevance to pigmentation and melanoma biology, and further emphasize the critical role of MITF in these processes.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Dermatology Clinic
DDC:610 Medicine & health
Language:English
Date:December 2008
Deposited On:26 Feb 2009 10:55
Last Modified:27 Nov 2013 19:51
Publisher:Wiley-Blackwell
ISSN:1755-1471
Publisher DOI:10.1111/j.1755-148X.2008.00505.x
PubMed ID:19067971
Citations:Web of Science®. Times Cited: 58
Google Scholar™

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page