Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive 

Ehnes, C; Forster, I C; Köhler, K; Biber, J; Murer, H (2002). Functional studies on a split type II Na/P(i)-cotransporter. Journal of Membrane Biology, 118(3):227-236.

Full text not available from this repository.

Abstract

Analysis of rat and mouse proximal tubular brush-border membrane expression of the type IIa Na/P(i)-cotransporter provides evidence for its cleavage in the large extracellular loop (ECL-2). To study functional properties and membrane distribution of this split NaP(i)-IIa transporter we followed two strategies. In one strategy we expressed the transporter as two complementary parts (p40 and p45) in Xenopus laevis oocytes and as another strategy we cleaved the WT protein with trypsin. Both strategies resulted in a split NaP(i)-IIa protein located in the plasma membrane. The two domains were tied together by a disulfide bridge, most likely involving the cysteines 306 and 334. Surface expression of the NaP(i)-IIa fragments was dependent on the presence of both domains. If both domains were coexpressed, the transporter was functional and transport characteristics were identical to those of the WT-NaP(i)-IIa protein. Corresponding to this, the transporter cleaved by trypsin also retains its transport capacity. These data indicate that cleavage of the type IIa Na/P(i)-cotransporter at ECL-2 is compatible with its cotransport function.

Item Type:Journal Article, refereed
Communities & Collections:04 Faculty of Medicine > Institute of Physiology
07 Faculty of Science > Institute of Physiology
DDC:570 Life sciences; biology
Language:English
Date:1 August 2002
Deposited On:11 Feb 2008 12:22
Last Modified:28 Nov 2013 00:16
Publisher:Springer
ISSN:0022-2631
Publisher DOI:10.1007/s00232-001-0186-y
PubMed ID:12181613
Citations:Web of Science®. Times Cited: 10
Google Scholar™

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page