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Forster, I C; Köhler, K; Stange, G; Biber, J; Murer, H (2002). Modulation of renal type IIa Na+/Pi cotransporter kinetics by the arginine modifier phenylglyoxal. Journal of Membrane Biology, 187(2):85-96.

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Abstract

The effects of the arginine-modifying reagent phenylglyoxal on the kinetics of the type IIa Na + /Pi cotransporter expressed in Xenopus, oocytes were studied by means of 32Pi uptake and electrophysiology. Phenylglyoxal incubation induced up to 60% loss of cotransport function but only marginally altered the Na+-leak. Substrate activation and pH dependency remained essentially unaltered, whereas the voltage dependency of Pi-induced change in electrogenic response was significantly reduced. Presteady-state charge movements were suppressed and the equilibrium charge distribution was shifted slightly towards hyperpolarizing potentials. Charge movements in the absence of external Na+ were also suppressed, which indicated that the empty-carrier kinetics were modified. These effects were incorporated into an ordered alternating access model for NaPi-IIa, whereby the arginine modification by phenylglyoxal was modeled as altered apparent electrical distances moved by mobile charges, together with a slower rate of translocation of the electroneutral, fully loaded carrier.

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Additional indexing

Item Type:Journal Article, refereed
Communities & Collections:04 Faculty of Medicine > Institute of Physiology
07 Faculty of Science > Institute of Physiology
DDC:570 Life sciences; biology
Language:English
Date:15 May 2002
Deposited On:11 Feb 2008 12:23
Last Modified:27 Nov 2013 16:52
Publisher:Springer
ISSN:0022-2631
Publisher DOI:10.1007/s00232-001-0154-6
PubMed ID:12029367

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