Moschèn, I; Setiawan, I; Bröer, S; Murer, H; Lang, F (2001). Effect of NaPi-mediated phosphate transport on intracellular pH. Pflügers Archiv: European Journal of Physiology (Pflugers Archiv), 441(6):802-806.
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Extracellular pH has been shown previously to influence transport via type-II Na+/phosphate (NaPi) transporters by modifying the affinity of the carrier for Na+ and by altering the availability of divalent and monovalent phosphate. As the transport of monovalent phosphate would be expected to acidify, and that of divalent phosphate to alkalinize the cell interior, the effect of phosphate transport on cytosolic pH was studied using ion selective microelectrodes in Xenopus oocytes expressing NaPi-3 or NaPi-5. At an alkaline extracellular pH (pHe) of 8.0, addition of phosphate elicited a strong inward current, depolarization of the cell membrane and cytosolic alkalinization. At pHe 6.0 the phosphate-induced inward current and depolarization were reduced and the alkalinization completely abolished. In conclusion, at alkaline pHe phosphate transport is enhanced and the transport of divalent phosphate prevails. At pHe 6.0, phosphate transport is attenuated and is accomplished by transport of both divalent and monovalent phosphate.
|Item Type:||Journal Article, refereed|
|Communities & Collections:||04 Faculty of Medicine > Institute of Physiology|
07 Faculty of Science > Institute of Physiology
|DDC:||570 Life sciences; biology|
|Date:||01 March 2001|
|Deposited On:||11 Feb 2008 13:23|
|Last Modified:||27 Nov 2013 23:13|
|Citations:||Web of Science®. Times cited: 7|
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