Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-14315

Quesada, P; Malanga, M; Di Meglio, S; De Lorenzo, S; Fabbrocini, A; Garbi, C; Bianco, A R; Pepe, S (2003). Recombinant IFN-alpha2b treatment activates poly (ADPR) polymerase-1 (PARP-1) in KB cancer cells. European Journal of Cancer, 39(14):2103-2109.

[img] PDF - Registered users only
1MB

View at publisher

Abstract

In the present paper, we investigated the relationship between the growth inhibitory effects of recombinant interferon-alpha2b (rIFN-alpha2b) and poly (ADPR) polymerase-1 (PARP-1) activity in the human squamous KB cancer cell line. Growth inhibition of the KB cells mediated by 1000 IU/ml of rIFN-alpha2b was accompanied by a transient rise in PARP-1 specific activity 24 h after rIFN-alpha2b treatment, confirmed by both the increase of intracellular poly (ADP-ribose) content and the PARP-1 auto-modification level. At longer times of incubation, the onset of apoptosis accompanied KB cell growth inhibition, as demonstrated by both flow cytometry and western-blotting analysis showing an 89 kDa apoptotic fragment of PARP-1. Moreover, pretreatment of the cells with the PARP-1 inhibitor, 3-aminobenzamide (3-ABA), at non-cytotoxic concentrations (1 mM), reduced the cell-growth inhibition, cell-cycle perturbation and apoptosis caused by rIFN-alpha2b. Taken together, these results strongly suggest that PARP-1 may be directly involved in the effects of rIFN-alpha2b in the KB cancer cell line.

Citations

Altmetrics

Downloads

1 download since deposited on 20 Mar 2009
0 downloads since 12 months

Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Veterinary Pharmacology and Toxicology
DDC:570 Life sciences; biology
Language:English
Date:2003
Deposited On:20 Mar 2009 13:00
Last Modified:23 Nov 2012 14:19
Publisher:Elsevier
ISSN:0959-8049
Funders:others
Publisher DOI:10.1016/S0959-8049(03)00433-7
PubMed ID:12957467

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page