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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-14373

Petrushanko, I Y; Bogdanov, N B; Lapina, N; Boldyrev, A A; Gassmann, M; Bogdanova, A (2007). Oxygen-induced regulation of Na/K ATPase in cerebellar granule cells. Journal of General Physiology, 130(4):389-398.

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Abstract

Adjustment of the Na/K ATPase activity to changes in oxygen availability is a matter of survival for neuronal cells. We have used freshly isolated rat cerebellar granule cells to study oxygen sensitivity of the Na/K ATPase function. Along with transport and hydrolytic activity of the enzyme we have monitored alterations in free radical production, cellular reduced glutathione, and ATP levels. Both active K(+) influx and ouabain-sensitive inorganic phosphate production were maximal within the physiological pO(2) range of 3-5 kPa. Transport and hydrolytic activity of the Na/K ATPase was equally suppressed under hypoxic and hyperoxic conditions. The ATPase response to changes in oxygenation was isoform specific and limited to the alpha1-containing isozyme whereas alpha2/3-containing isozymes were oxygen insensitive. Rapid activation of the enzyme within a narrow window of oxygen concentrations did not correlate with alterations in the cellular ATP content or substantial shifts in redox potential but was completely abolished when NO production by the cells was blocked by l-NAME. Taken together our observations suggest that NO and its derivatives are involved in maintenance of high Na/K ATPase activity under physiological conditions.

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13 citations in Web of Science®
16 citations in Scopus®
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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Veterinary Physiology
04 Faculty of Medicine > Center for Integrative Human Physiology
DDC:570 Life sciences; biology
610 Medicine & health
Language:English
Date:October 2007
Deposited On:20 Mar 2009 10:04
Last Modified:27 Nov 2013 19:42
Publisher:Rockefeller University Press
ISSN:0022-1295
Publisher DOI:10.1085/jgp.200709783
PubMed ID:17893192

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