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Application of pulsed magnetic field enhances non-viral gene delivery in primary cells from different origins


Kamau Chapman, S; Hassa, P; Koch, S; von Rechenberg, Brigitte; Hofmann-Amtenbrink, M; Steitz, B; Petri-Fink, A; Hofmann, H; Hottiger, M O (2008). Application of pulsed magnetic field enhances non-viral gene delivery in primary cells from different origins. Journal of Magnetism and Magnetic Materials, 320(8):1517-1527.

Abstract

Primary cell lines are more difficult to transfect when compared to immortalized/transformed cell lines, and hence new techniques are required to enhance the transfection efficiency in these cells. We isolated and established primary cultures of synoviocytes, chondrocytes, osteoblasts, melanocytes, macrophages, lung fibroblasts, and embryonic fibroblasts. These cells differed in several properties, and hence were a good representative sample of cells that would be targeted for expression and delivery of therapeutic genes in vivo. The efficiency of gene delivery in all these cells was enhanced using polyethylenimine-coated polyMAG magnetic nanoparticles, and the rates (17–84.2%) surpassed those previously achieved using other methods, especially in cells that are difficult to transfect. The application of permanent and pulsating magnetic fields significantly enhanced the transfection efficiencies in synoviocytes, chondrocytes, osteoblasts, melanocytes and lung fibroblasts, within 5 min of exposure to these magnetic fields. This is an added advantage for future in vivo applications, where rapid gene delivery is required before systemic clearance or filtration of the gene vectors occurs.

Primary cell lines are more difficult to transfect when compared to immortalized/transformed cell lines, and hence new techniques are required to enhance the transfection efficiency in these cells. We isolated and established primary cultures of synoviocytes, chondrocytes, osteoblasts, melanocytes, macrophages, lung fibroblasts, and embryonic fibroblasts. These cells differed in several properties, and hence were a good representative sample of cells that would be targeted for expression and delivery of therapeutic genes in vivo. The efficiency of gene delivery in all these cells was enhanced using polyethylenimine-coated polyMAG magnetic nanoparticles, and the rates (17–84.2%) surpassed those previously achieved using other methods, especially in cells that are difficult to transfect. The application of permanent and pulsating magnetic fields significantly enhanced the transfection efficiencies in synoviocytes, chondrocytes, osteoblasts, melanocytes and lung fibroblasts, within 5 min of exposure to these magnetic fields. This is an added advantage for future in vivo applications, where rapid gene delivery is required before systemic clearance or filtration of the gene vectors occurs.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Veterinary Biochemistry and Molecular Biology
05 Vetsuisse Faculty > Veterinary Clinic > Equine Department
Dewey Decimal Classification:570 Life sciences; biology
Uncontrolled Keywords:Nanoparticle ; Permanent magnet ; Magnetic particles ; Gene ; Cell ; Primary culture ; Magnetic field effect ; Pulsed field
Language:English
Date:April 2008
Deposited On:19 Feb 2009 15:54
Last Modified:04 Jun 2016 07:24
Publisher:Elsevier
ISSN:0304-8853
Publisher DOI:https://doi.org/10.1016/j.jmmm.2008.01.002
Permanent URL: https://doi.org/10.5167/uzh-15748

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