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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-16068

Feng, J; Fischer, G; Lucchinetti, E; Zhu, M; Bestmann, L; Jegger, D; Arras, M; Pasch, T; Perriard, J C; Schaub, M C; Zaugg, M (2006). Infarct-remodeled myocardium is receptive to protection by isoflurane postconditioning: role of protein kinase B/Akt signaling. Anesthesiology, 104(5):1004-1014.

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Abstract

BACKGROUND: Postinfarct remodeled myocardium exhibits numerous structural and biochemical alterations. So far, it is unknown whether postconditioning elicited by volatile anesthetics can also provide protection in the remodeled myocardium. METHODS: Myocardial infarct was induced in male Wistar rats by ligation of the left anterior descending coronary artery. Six weeks later, hearts were buffer-perfused and exposed to 40 min of ischemia followed by 90 min of reperfusion. Anesthetic postconditioning was induced by 15 min of 2.1 vol% isoflurane. In some experiments, LY294002 (15 microM), a phosphatidylinositol 3-kinase inhibitor, was coadministered with isoflurane. Masson's trichrome staining, immunohistochemistry, Western blot analysis, and reverse-transcription polymerase chain reaction served to confirm remodeling. In buffer-perfused hearts, functional recovery was recorded, and acute infarct size was measured using 1% triphenyltetrazolium chloride staining and lactate dehydrogenase release during reperfusion. Western blot analysis was used to determine phosphorylation of reperfusion injury salvage kinases including protein kinase B/Akt and its downstream targets after 15 min of reperfusion. RESULTS: Infarct hearts exhibited typical macroscopic and molecular changes of remodeling. Isoflurane postconditioning improved functional recovery and decreased acute infarct size, as determined by triphenyltetrazolium (35 +/- 5% in unprotected hearts vs. 8 +/- 3% in anesthetic postconditioning; P < 0.05) and lactate dehydrogenase release. This protection was abolished by LY294002, which inhibited phosphorylation of protein kinase B/Akt and its downstream targets glycogen synthase kinase 3beta, endothelial nitric oxide synthase, and p70S6 kinase. CONCLUSIONS: Infarct-remodeled myocardium is receptive to protection by isoflurane postconditioning via protein kinase B/Akt signaling. This is the first time to demonstrate that anesthetic postconditioning retains its marked protection in diseased myocardium.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Pharmacology and Toxicology
04 Faculty of Medicine > Center for Integrative Human Physiology
05 Vetsuisse Faculty > Institute of Laboratory Animal Science
04 Faculty of Medicine > Institute of Laboratory Animal Science

04 Faculty of Medicine > University Hospital Zurich > Institute of Anesthesiology
DDC:570 Life sciences; biology
610 Medicine & health
Language:English
Date:May 2006
Deposited On:18 Mar 2009 20:03
Last Modified:28 Nov 2013 02:01
Publisher:Lippincott Wiliams & Wilkins
ISSN:0003-3022
Publisher DOI:10.1097/00000542-200605000-00017
Official URL:http://journals.lww.com/anesthesiology/pages/articleviewer.aspx?year=2006&issue=05000&article=00017&type=abstract
PubMed ID:16645453
Citations:Web of Science®. Times Cited: 52
Google Scholar™
Scopus®. Citation Count: 57

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