Quick Search:

uzh logo
Browse by:

Zurich Open Repository and Archive

Maintenance: Tuesday, 5.7.2016, 07:00-08:00

Maintenance work on ZORA and JDB on Tuesday, 5th July, 07h00-08h00. During this time there will be a brief unavailability for about 1 hour. Please be patient.

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-16083

Kappeler, M; Kranz, E; Woolcock, K; Georgiev, O; Schaffner, W (2008). Drosophila bloom helicase maintains genome integrity by inhibiting recombination between divergent DNA sequences. Nucleic Acids Research, 36(21):6907-6917.

View at publisher


DNA double strand breaks (DSB) can be repaired either via a sequence independent joining of DNA ends or via homologous recombination. We established a detection system in D. melanogaster to investigate the impact of sequence constraints on the usage of the homology based DSB repair via single strand annealing (SSA), which leads to recombination between direct repeats with concomitant loss of one repeat copy. First of all, we find the SSA frequency to be inversely proportional to the spacer length between the repeats, for spacers up to 2.4 kb in length. We further show that SSA between divergent repeats (homeologous SSA) is suppressed in cell cultures and in vivo in a sensitive manner, recognizing sequence divergences smaller than 0.5%. Finally, we demonstrate that the suppression of homeologous SSA depends on the Bloom helicase (Blm), encoded by the Drosophila gene mus309. Suppression of homeologous recombination is a novel function of Blm in ensuring genomic integrity, not described to date in mammalian systems. Unexpectedly, distinct from its function in S. cerevisiae, the mismatch repair (MMR) factor Msh2 encoded by spel1 does not suppress homeologous SSA in Drosophila.


9 citations in Web of Science®
9 citations in Scopus®
Google Scholar™



123 downloads since deposited on 02 Mar 2009
18 downloads since 12 months

Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Molecular Life Sciences
Dewey Decimal Classification:570 Life sciences; biology
Date:December 2008
Deposited On:02 Mar 2009 16:06
Last Modified:05 Apr 2016 13:05
Publisher:Oxford University Press
Funders:Schweizerischer Nationalfonds, project “Controlled Gene Integration” (LSHG-CT-2003-503303), Kanton Zürich
Additional Information:Full final text Oxford Journal
Publisher DOI:10.1093/nar/gkn793
PubMed ID:18978019

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page