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Giertsen, E; Guggenheim, B; Thurnheer, T; Gmür, R (2000). Microbiological aspects of an in situ model to study effects of antimicrobial agents on dental plaque ecology. European Journal of Oral Sciences, 108(5):403-411.

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Abstract

This study validates an in situ model for ecological studies of dental plaque exposed to various antimicrobial agents with different modes of action on plaque bacteria. Eleven subjects wore two acrylic appliances, each containing two bovine enamel discs, during two 1-wk test periods. Using a split-mouth crossover design, the appliances were dipped twice daily for 1 min into water (control; treatment A), fluoride (26.3 mM NaF; B), zinc acetate (20.0 mM; C), or fluoride plus zinc acetate (D). Four of the subjects used also chlorhexidine diacetate (2.2 mM; E) and chlorhexidine plus fluoride (F). At the end of each period, plaque was collected from the discs, after which the microbiota were analyzed by culture, automated quantitative immunofluorescence, and a viability fluorescence stain. As compared to control, treatments B, C, and D resulted in a significant reduction of individual taxa as detected by immunofluorescence, whereas similar bacterial viability and total bacterial numbers were observed. In contrast, chlorhexidine significantly reduced bacterial viability, total cell numbers, and the abundance of most of the enumerated taxa. We conclude that this in situ model is well suited to study effects of antimicrobial agents on dental plaque ecology. Combined with viability testing, immunofluorescence is obviously superior to culture in detecting taxa-specific shifts caused by antimicrobial agents.

Item Type:Journal Article, refereed
Communities & Collections:04 Faculty of Medicine > Center for Dental Medicine > Institute of Oral Biology
DDC:610 Medicine & health
Language:English
Date:1 October 2000
Deposited On:11 Feb 2008 12:24
Last Modified:28 Nov 2013 01:22
Publisher:Wiley-Blackwell
ISSN:0909-8836
Publisher DOI:10.1034/j.1600-0722.2000.108005403.x
PubMed ID:11037756
Citations:Web of Science®. Times Cited: 13
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