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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-16604

Port, F; Kuster, M; Herr, P; Furger, E; Bänziger, C; Hausmann, G; Basler, K (2008). Wingless secretion promotes and requires retromer-dependent cycling of Wntless. Nature Cell Biology, 10(2):178-185.

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Abstract

Wnt ligands are lipid-modified, secreted glycoproteins that control multiple steps during embryogenesis and adult-tissue homeostasis. Little is known about the mechanisms underlying Wnt secretion. Recently, Wntless (Wls/Evi/Srt) was identified as a conserved multi-pass transmembrane protein whose function seems to be dedicated to promoting the release of Wnts. Here, we describe Wls accumulation in the Golgi apparatus of Wnt/Wingless (Wg)-producing cells in Drosophila, and show that this localization is essential for Wg secretion. Moreover, Wls localization and levels critically depend on retromer, a conserved protein complex that mediates endosome-to-Golgi protein trafficking in yeast. In the absence of the retromer components Dvps35 or Dvps26, but in presence of Wg, Wls is degraded and Wg secretion impaired. Our results indicate that Wg, clathrin-mediated endocytosis and retromer sustain a Wls traffic loop from the Golgi to the plasma membrane and back to the Golgi, thereby enabling Wls to direct Wnt secretion.

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Molecular Life Sciences
DDC:570 Life sciences; biology
Language:English
Date:2008
Deposited On:06 Mar 2009 10:22
Last Modified:04 Dec 2013 04:58
Publisher:Nature Publishing Group
ISSN:1465-7392
Publisher DOI:10.1038/ncb1687
PubMed ID:18193032
Citations:Web of Science®. Times Cited: 93
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