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Recombinases and their use in gene activation, gene inactivation, and transgenesis


Bischof, J; Basler, K (2008). Recombinases and their use in gene activation, gene inactivation, and transgenesis. In: Dahmann, C. Drosophila : methods and protocols. Totowa, N.J., US: Springer, 175-195.

Abstract

The site-specific recombinase FLP is used in Drosophila to precisely manipulate the genome, in particular, to eliminate gene function by mitotic recombination and to activate transgenes in discrete populations of cells. These approaches are already part of the standard tool kit for studying gene function. The number of applications for the FLP recombinase has increased over the years and further members of the large family of site-specific recombinases are being added to the arsenal of fly geneticists, most recently, the phiC31 integrase. This chapter will introduce these recombinases and describe how such instruments are utilized to accurately manipulate the Drosophila genome.

The site-specific recombinase FLP is used in Drosophila to precisely manipulate the genome, in particular, to eliminate gene function by mitotic recombination and to activate transgenes in discrete populations of cells. These approaches are already part of the standard tool kit for studying gene function. The number of applications for the FLP recombinase has increased over the years and further members of the large family of site-specific recombinases are being added to the arsenal of fly geneticists, most recently, the phiC31 integrase. This chapter will introduce these recombinases and describe how such instruments are utilized to accurately manipulate the Drosophila genome.

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Additional indexing

Item Type:Book Section, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Molecular Life Sciences
Dewey Decimal Classification:570 Life sciences; biology
Language:English
Date:2008
Deposited On:02 Mar 2009 19:08
Last Modified:05 Apr 2016 13:07
Publisher:Springer
Series Name:Methods in Molecular Biology
Number:420
ISSN:1064-3745
ISBN:978-1-58829-817-1
Publisher DOI:10.1007/978-1-59745-583-1_10
PubMed ID:18641947
Permanent URL: http://doi.org/10.5167/uzh-16605

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