UZH-Logo

A captured viral interleukin 10 gene with cellular exon structure


Jayawardane, G; Russel, G C; Thomson, J; Deane, D; Cox, H; Gatherer, D; Ackermann, M; Haig, D M; Stewart, J P (2008). A captured viral interleukin 10 gene with cellular exon structure. Journal of General Virology, 89(10):2447-2455.

Abstract

We have characterized a novel, captured and fully functional viral interleukin (IL)-10 homologue (OvHVIL-10) from the gammaherpesvirus ovine herpesvirus 2. Unlike IL-10 homologues from other gammaherpesviruses, the OvHVIL-10 peptide sequence was highly divergent from that of the host
species. The OvHVIL-10 gene is unique amongst virus captured genes in that it has precisely retained the original cellular exon structure, having five exons of similar sizes to the cellular counterparts. However, the sizes of the introns are dramatically reduced. The OvHVIL-10 protein
was shown to be a non-glycosylated, secreted protein of Mr 21 000 with a signal peptidase cleavage site between amino acids 26 and 27 of the nascent peptide. Functional assays showed that OvHVIL-10, in a similar way to ovine IL-10, stimulated mast cell proliferation and inhibited macrophage inflammatory chemokine production. This is the first example of a captured herpesvirus gene retaining the full cellular gene structure.

We have characterized a novel, captured and fully functional viral interleukin (IL)-10 homologue (OvHVIL-10) from the gammaherpesvirus ovine herpesvirus 2. Unlike IL-10 homologues from other gammaherpesviruses, the OvHVIL-10 peptide sequence was highly divergent from that of the host
species. The OvHVIL-10 gene is unique amongst virus captured genes in that it has precisely retained the original cellular exon structure, having five exons of similar sizes to the cellular counterparts. However, the sizes of the introns are dramatically reduced. The OvHVIL-10 protein
was shown to be a non-glycosylated, secreted protein of Mr 21 000 with a signal peptidase cleavage site between amino acids 26 and 27 of the nascent peptide. Functional assays showed that OvHVIL-10, in a similar way to ovine IL-10, stimulated mast cell proliferation and inhibited macrophage inflammatory chemokine production. This is the first example of a captured herpesvirus gene retaining the full cellular gene structure.

Citations

18 citations in Web of Science®
17 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

1 download since deposited on 25 Feb 2009
0 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Virology
Dewey Decimal Classification:570 Life sciences; biology
Language:English
Date:2008
Deposited On:25 Feb 2009 13:52
Last Modified:05 Apr 2016 13:07
Publisher:Society for General Microbiology
ISSN:0022-1317
Publisher DOI:10.1099/vir.0.2008/001743-0
Permanent URL: http://doi.org/10.5167/uzh-16630

Download

[img]
Filetype: PDF - Registered users only
Size: 278kB
View at publisher

TrendTerms

TrendTerms displays relevant terms of the abstract of this publication and related documents on a map. The terms and their relations were extracted from ZORA using word statistics. Their timelines are taken from ZORA as well. The bubble size of a term is proportional to the number of documents where the term occurs. Red, orange, yellow and green colors are used for terms that occur in the current document; red indicates high interlinkedness of a term with other terms, orange, yellow and green decreasing interlinkedness. Blue is used for terms that have a relation with the terms in this document, but occur in other documents.
You can navigate and zoom the map. Mouse-hovering a term displays its timeline, clicking it yields the associated documents.

Author Collaborations