Bourikas, Dimitris; Pekarik, Vladimir; Baeriswyl, T; Grunditz, A; Sadhu, R; Nardó, M; Stoeckli, E T (2005). Sonic hedgehog guides commissural axons along the longitudinal axis of the spinal cord. Nature Neuroscience, 8(3):297-304.
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Dorsal commissural axons in the developing spinal cord cross the floor plate, then turn rostrally and grow along the longitudinal axis, close to the floor plate. We used a subtractive hybridization approach to identify guidance cues responsible for the rostral turn in chicken embryos. One of the candidates was the morphogen Sonic hedgehog (Shh). Silencing of the gene SHH (which encodes Shh) by in ovo RNAi during commissural axon navigation demonstrated a repulsive role in post-commissural axon guidance. This effect of Shh was not mediated by Patched (Ptc) and Smoothened (Smo), the receptors that mediate effects of Shh in morphogenesis and commissural axon growth toward the floor plate. Rather, functional in vivo studies showed that the repulsive effect of Shh on postcommissural axons was mediated by Hedgehog interacting protein (Hip).
|Item Type:||Journal Article, refereed|
|Communities & Collections:||07 Faculty of Science > Institute of Molecular Life Sciences|
|DDC:||570 Life sciences; biology|
|Date:||01 March 2005|
|Deposited On:||11 Feb 2008 13:13|
|Last Modified:||27 Nov 2013 19:13|
|Publisher:||Nature Publishing Group|
|Citations:||Web of Science®. Times cited: 106|
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