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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-17352

Garas, M; Dichtl, B; Keller, W (2008). The role of the putative 3' end processing endonuclease Ysh1p in mRNA and snoRNA synthesis. RNA, 14(12):2671-2684.

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Abstract

Pre-mRNA 3' end formation is tightly linked to upstream and downstream events of eukaryotic mRNA synthesis. The two-step reaction involves endonucleolytic cleavage of the primary transcript followed by poly(A) addition to the upstream cleavage product. To further characterize the putative 3' end processing endonuclease Ysh1p/Brr5p, we isolated and analyzed a number of new temperature- and cold-sensitive mutant alleles. We show that Ysh1p plays a crucial role in 3' end formation and in RNA polymerase II (RNAP II) transcription termination on mRNA genes. In addition, we observed a range of additional functional deficiencies in ysh1 mutant strains, which were partially allele-specific. Interestingly, snoRNA 3' end formation and RNAP II termination were defective on specific snoRNAs in the cold-sensitive ysh1-12 strain. Moreover, we observed the accumulation of several mRNAs including the NRD1 transcript in this mutant. We provide evidence that NRD1 autoregulation is associated with endonucleolytic cleavage and that this process may involve Ysh1p. In addition, the ysh1-12 strain displayed defects in RNA splicing indicating that a functional link may exist between intron removal and 3' end formation in yeast. These observations suggest that Ysh1p has multiple roles in RNA synthesis and processing.

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Molecular Life Sciences
DDC:570 Life sciences; biology
Language:English
Date:December 2008
Deposited On:07 Mar 2009 17:52
Last Modified:27 Nov 2013 19:17
Publisher:Cold Spring Harbor Laboratory Press
ISSN:1355-8382
Publisher DOI:10.1261/rna.1293008
PubMed ID:18971324
Citations:Web of Science®. Times Cited: 12
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Scopus®. Citation Count: 12

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