Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-178
Rinner, O; Rick, J M; Neuhauss, S C F (2005). Contrast sensitivity, spatial and temporal tuning of the larval zebrafish optokinetic response. Investigative Ophthalmology and Visual Science, 46(1):137-142.
PURPOSE: To characterize the quantitative properties of the optokinetic response (OKR) in zebrafish larvae as a tool to test visual performance in genetically modified larvae. METHODS: Horizontal OKR was triggered in 5-day-old zebrafish larvae by stimulation with projected computer-generated gratings of varying contrast, angular velocity, temporal and spatial frequency, and brightness. Eye movements were analyzed by a custom-made eye tracker based on image analysis. RESULTS: The gain of the OKR slow phase was dependent on angular velocity, spatial frequency, and contrast of a moving grating, but largely independent on brightness. Eye velocity was a logarithmically linear function of grating contrast with a slope of approximately 0.8 per log unit contrast. CONCLUSIONS: The OKR of the larval zebrafish is not scaled for stimulus contrast and spatial frequency. These properties make the OKR a valuable tool to quantify behavioral visual performance such as visual acuity, contrast sensitivity, and light adaptation. This behavioral paradigm will be useful for analyzing visual performance in mutant and gene-knockdown larval zebrafish.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||07 Faculty of Science > Institute of Molecular Life Sciences|
|DDC:||570 Life sciences; biology|
|Date:||1 January 2005|
|Deposited On:||11 Feb 2008 12:13|
|Last Modified:||17 Jul 2014 03:15|
|Publisher:||Association for Research in Vision and Ophthalmology|
|Citations:||Web of Science®. Times Cited: 44|
Scopus®. Citation Count: 52
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