Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-18248
Baici, A; Schenker, P; Wächter, M; Rüedi, P (2009). 3-Fluoro-2,4-dioxa-3-phosphadecalins as inhibitors of acetylcholinesterase: a reappraisal of kinetic mechanisms and diagnostic methods. Chemistry & Biodiversity, 6(3):261-282.
- Registered users only
View at publisher
A systematic survey of the acetylcholine-mimetic 2,4-dioxa-3-phosphadecalins as irreversible inhibitors of acetylcholinesterase revealed hitherto overlooked properties as far as the kinetic mechanisms of interaction are concerned. As a support to past and future work in this field, we describe the kinetics of eight reaction schemes that may be found in irreversible enzyme modification and compare them with two mechanism of reversible, slow-binding inhibition. The relevant kinetic equations and their associated graphical representations are given for all mechanisms, and concrete examples illustrate their practical use. Since irreversible inhibition is a time-dependent phenomenon, kinetic analysis is greatly facilitated by fitting the appropriate integrated rate equations to reaction-progress curves by nonlinear regression. This primary scrutiny provides kinetic parameters that are indispensable tools for diagnosing the kinetic mechanism and for calculating inhibition constants. Numerical integration of sets of differential equations is an additional useful investigation tool in critical situations, e.g., when inhibitors are unstable and/or act as irreversible modifiers only temporarily.
1 download since deposited on 16 Apr 2009
0 downloads since 12 months
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > Department of Biochemistry
07 Faculty of Science > Department of Biochemistry
|Dewey Decimal Classification:||570 Life sciences; biology|
|Deposited On:||16 Apr 2009 10:15|
|Last Modified:||05 Apr 2016 13:12|
|Publisher:||Verlag Helvetica Chimica Acta|
Users (please log in): suggest update or correction for this item
Repository Staff Only: item control page