Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-1828
Cathomen, T; Mrkic, B; Spehner, D; Drillien, R; Naef, R; Pavlovic, J; Aguzzi, A; Billeter, M A; Cattaneo, R (1998). A matrix-less measles virus is infectious and elicits extensive cell fusion: consequences for propagation in the brain. The EMBO Journal, 17(14):3899-3908.
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Measles viruses (MV) can be isolated from the brains of deceased subacute sclerosing panencephalitis patients only in a cell-associated form. These viruses are often defective in the matrix (M) protein and always seem to have an altered fusion protein cytoplasmic tail. We reconstituted a cell-free, infectious M-less MV (MV-DeltaM) from cDNA. In comparison with standard MV, MV-DeltaM was considerably more efficient at inducing cell-to-cell fusion but virus titres were reduced approximately 250-fold. In MV-DeltaM-induced syncytia the ribonucleocapsids and glycoproteins largely lost co-localization, confirming the role of M protein as the virus assembly organizer. Genetically modified mice were inoculated with MV-DeltaM or with another highly fusogenic virus bearing glycoproteins with shortened cytoplasmic tails (MV-Delta(tails)). MV-DeltaM and MV-Delta(tails) lost acute pathogenicity but penetrated more deeply into the brain parenchyma than standard MV. We suggest that enhanced cell fusion may also favour the propagation of mutated, assembly-defective MV in human brains.
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|Item Type:||Journal Article, refereed|
|Communities & Collections:||04 Faculty of Medicine > University Hospital Zurich > Institute of Neuropathology|
|Dewey Decimal Classification:||570 Life sciences; biology
610 Medicine & health
|Date:||15 July 1998|
|Deposited On:||11 Feb 2008 12:25|
|Last Modified:||27 Nov 2013 18:20|
|Publisher:||European Molecular Biology Organization ; Nature Publishing Group|
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