Heinzer, S; Krucker, T; Stampanoni, M; Abela, R; Meyer, E P; Schuler, A; Schneider, P; Müller, R (2004). Hierarchical bioimaging and quantification of vasculature in disease models using corrosion casts and microcomputed tomography. Proceedings of SPIE, 5535:65-76.
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A wide range of disorders are associated with alterations of the central and peripheral vascular system. Modified vascular corrosion casting using a newly developed polymer, allows for the first time hierarchical assessment of 3D vessel data in animals down to the level of capillaries. Imaging of large volumes of vasculature at intermediate resolution (16 um) was performed using a desktop micro-computed tomography system. Subsequently regions of interest were identified for additional high resolution imaging (1.4 um) at the X-ray Tomographic Microscopy (XTM) station of the Swiss Light Source (SLS). A framework for systematic hierarchical imaging and quantification was developed. Issues addressed included enhanced XTM data acquisition, introduction of local tomography, sample navigation, advanced post processing, and data combination. In addition to visual assessment of qualitative changes, morphometrical and architectural indices were determined using direct 3D morphometry software developed in house. Vessel specific parameters included thickness, surface, connectivity, and vessel length. Reconstructions of cerebral vasculature in mutant mice modeling Alzheimer's disease revealed significant changes in vessel architecture and morphology. In the future, a combination of these techniques may support drug discovery. Additionally, future ultra-high-resolution in vivo systems may even allow non-invasive tracking of temporal alterations in vascular morphology.
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|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||07 Faculty of Science > Institute of Zoology (former)|
|DDC:||570 Life sciences; biology
590 Animals (Zoology)
|Date:||2 November 2004|
|Deposited On:||11 Feb 2008 12:13|
|Last Modified:||27 Nov 2013 22:46|
|Publisher:||SPIE - International Society for Optical Engineering|
|Additional Information:||Copyright 2004 Society of Photo-Optical Instrumentation Engineers. This paper was published in Proceedings of SPIE and is made available as an electronic reprint with permission of SPIE. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.|
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