Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive

Maintenance: Tuesday, 5.7.2016, 07:00-08:00

Maintenance work on ZORA and JDB on Tuesday, 5th July, 07h00-08h00. During this time there will be a brief unavailability for about 1 hour. Please be patient.

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-1842

Zanusso, G; Liu, D; Ferrari, S; Hegyi, I; Yin, X; Aguzzi, A; Hornemann, S; Liemann, S; Glockshuber, R; Manson, J C; Brown, P; Petersen, R B; Gambetti, P; Sy, M S (1998). Prion protein expression in different species: analysis with a panel of new mAbs. Proceedings of the National Academy of Sciences of the United States of America (PNAS), 95(15):8812-8816.

[img]
Preview
PDF
310kB
View at publisher
[img]
Preview
PDF
310kB

Abstract

By immunizing prion knockout mice (Prnp-/-) with recombinant murine prion protein (PrPc), we obtained a panel of mAbs specific for murine PrPc. These mAbs can be applied to immunoblotting, cell surface immunofluorescent staining, and immunohistochemistry at light and electron microscopy. These mAbs recognize both the normal (PrPc) and protease-resistant (PrPres) isoforms of PrP. Some mAbs are species restricted, while others react with PrP from a broad range of mammals including mice, humans, monkeys, cows, sheep, squirrels, and hamsters. Moreover, some of the mAbs selectively recognize different PrP glycoforms as well as the metabolic fragments of PrPc. These newly generated PrPc antibodies will help to explore the biology of PrPc and to establish the diagnosis of prion diseases in both humans and animals.

Citations

165 citations in Web of Science®
161 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

44 downloads since deposited on 11 Feb 2008
22 downloads since 12 months

Detailed statistics

Additional indexing

Item Type:Journal Article, refereed
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Institute of Neuropathology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:21 July 1998
Deposited On:11 Feb 2008 12:25
Last Modified:05 Apr 2016 12:20
Publisher:National Academy of Sciences
ISSN:0027-8424
Publisher DOI:10.1073/pnas.95.15.8812
Related URLs:http://www.pnas.org/cgi/content/abstract/95/15/8812
PubMed ID:9671761

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page