Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-1842
Zanusso, G; Liu, D; Ferrari, S; Hegyi, I; Yin, X; Aguzzi, A; Hornemann, S; Liemann, S; Glockshuber, R; Manson, J C; Brown, P; Petersen, R B; Gambetti, P; Sy, M S (1998). Prion protein expression in different species: analysis with a panel of new mAbs. Proceedings of the National Academy of Sciences of the United States of America (PNAS), 95(15):8812-8816.
By immunizing prion knockout mice (Prnp-/-) with recombinant murine prion protein (PrPc), we obtained a panel of mAbs specific for murine PrPc. These mAbs can be applied to immunoblotting, cell surface immunofluorescent staining, and immunohistochemistry at light and electron microscopy. These mAbs recognize both the normal (PrPc) and protease-resistant (PrPres) isoforms of PrP. Some mAbs are species restricted, while others react with PrP from a broad range of mammals including mice, humans, monkeys, cows, sheep, squirrels, and hamsters. Moreover, some of the mAbs selectively recognize different PrP glycoforms as well as the metabolic fragments of PrPc. These newly generated PrPc antibodies will help to explore the biology of PrPc and to establish the diagnosis of prion diseases in both humans and animals.
|Item Type:||Journal Article, refereed|
|Communities & Collections:||04 Faculty of Medicine > University Hospital Zurich > Institute of Neuropathology|
|DDC:||570 Life sciences; biology|
610 Medicine & health
|Date:||21 July 1998|
|Deposited On:||11 Feb 2008 12:25|
|Last Modified:||28 Nov 2013 00:46|
|Publisher:||National Academy of Sciences|
|Citations:||Web of Science®. Times Cited: 158|
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