Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-18537
Heimann, M; Käsermann, H P; Pfister, R; Roth, D R; Bürki, K (2009). Blood collection from the sublingual vein in mice and hamsters: a suitable alternative to retrobulbar technique that provides large volumes and minimizes tissue damage. Laboratory Animals, 43(3):255-206.
View at publisher
Blood examination is a key element in studies of laboratory animals. In rodents, retrobulbar venous plexus puncture is a commonly used method for obtaining a blood sample. Although this technique yields large volumes of blood, the disadvantage is that it can lead to severe tissue damage. The aim of the present study was to develop the puncture of V. sublingualis as a suitable alternative technique for drawing blood in mice and other rodents. In rats, this method has been established for collecting large blood volumes. During the first part of the study, the sublingual bleeding technique was developed for use in mice and hamsters. Guineapigs, however, do not have a sublingual vein; therefore, in this species the method is not possible. In the second part of the study, retrobulbar and sublingual methods were compared using male CD-1 mice. When compared with the retrobulbar method, sublingual venepuncture showed less tissue destruction in mice, with a decreased mean severity in the histological examination. In conclusion, sublingual venepuncture can be recommended as a suitable, alternative blood collection technique, because of the reduced risk of tissue damage in mice and hamsters.
192 downloads since deposited on 30 Apr 2009
25 downloads since 12 months
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||05 Vetsuisse Faculty > Institute of Laboratory Animal Science|
|Dewey Decimal Classification:||570 Life sciences; biology
610 Medicine & health
|Date:||23 February 2009|
|Deposited On:||30 Apr 2009 07:24|
|Last Modified:||27 Nov 2013 17:14|
|Publisher:||Royal Society of Medicine|
Users (please log in): suggest update or correction for this item
Repository Staff Only: item control page