Quick Search:

uzh logo
Browse by:

Zurich Open Repository and Archive

Maintenance: Tuesday, July the 26th 2016, 07:00-10:00

ZORA's new graphical user interface will be relaunched (For further infos watch out slideshow ZORA: Neues Look & Feel). There will be short interrupts on ZORA Service between 07:00am and 10:00 am. Please be patient.

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-18537

Heimann, M; Käsermann, H P; Pfister, R; Roth, D R; Bürki, K (2009). Blood collection from the sublingual vein in mice and hamsters: a suitable alternative to retrobulbar technique that provides large volumes and minimizes tissue damage. Laboratory Animals, 43(3):255-206.

View at publisher


Blood examination is a key element in studies of laboratory animals. In rodents, retrobulbar venous plexus puncture is a
commonly used method for obtaining a blood sample. Although this technique yields large volumes of blood, the
disadvantage is that it can lead to severe tissue damage. The aim of the present study was to develop the puncture of
V. sublingualis as a suitable alternative technique for drawing blood in mice and other rodents. In rats, this method has been established for collecting large blood volumes. During the first part of the study, the sublingual bleeding technique was developed for use in mice and hamsters. Guineapigs, however, do not have a sublingual vein; therefore, in this species the method is not possible. In the second part of the study, retrobulbar and sublingual methods were compared using male CD-1 mice. When compared with the retrobulbar method, sublingual venepuncture showed less tissue destruction in mice, with a
decreased mean severity in the histological examination. In conclusion, sublingual venepuncture can be recommended as a
suitable, alternative blood collection technique, because of the reduced risk of tissue damage in mice and hamsters.


25 citations in Web of Science®
27 citations in Scopus®
Google Scholar™



234 downloads since deposited on 30 Apr 2009
28 downloads since 12 months

Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Laboratory Animal Science
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Date:23 February 2009
Deposited On:30 Apr 2009 07:24
Last Modified:05 Apr 2016 13:13
Publisher:Royal Society of Medicine
Publisher DOI:10.1258/la.2008.007073
PubMed ID:19237457

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page