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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-18601

Al-Sabi, M N S; Kapel, C M O; Deplazes, P; Mathis, A (2007). Comparative copro-diagnosis of Echinococcus multilocularis in experimentally infected foxes. Parasitology Research, 101(3):731-736.

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Abstract

Faecal samples from 15 foxes experimentally infected with Echinococcus multilocularis were examined until 90 days post-infection (dpi) by microscopical identification of eggs isolated by flotation/sieving, by coproantigen-enzyme-linked immunosorbent assay (cELISA), by polymerase chain reaction (PCR) on DNA, respectively, isolated directly from the faecal samples (copro-DNA PCR) and from the eggs obtained by the flotation/sieving procedure (egg-DNA PCR). Based on egg counts, three periods of the infection were defined: pre-patent (2-29 dpi), high patent (30-70 dpi) and low patent periods (71-90 dpi). Whereas all methods were highly sensitive with samples from the high patent period, cELISA was the most sensitive to detect pre-patent infections (63%). Samples from the low patent infections were positive in 77% by microscopy and in 80% by egg-DNA PCR, being significantly more sensitive than cELISA and copro-DNA PCR. The isolation of eggs from the faecal material proved to be more sensitive by the flotation/sieving procedure as compared to the classical concentration McMaster technique.

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13 citations in Web of Science®
12 citations in Scopus®
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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Parasitology
04 Faculty of Medicine > Institute of Parasitology
DDC:570 Life sciences; biology
610 Medicine & health
600 Technology
Language:English
Date:2007
Deposited On:20 May 2009 08:36
Last Modified:02 Dec 2013 19:10
Publisher:Springer
ISSN:0932-0113
Additional Information:The original publication is available at www.springerlink.com
Publisher DOI:10.1007/s00436-007-0537-4
PubMed ID:17468972

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