Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive 

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-18926

Bentz, M; Döhner, H; Werner, C A; Huck, K; Baudis, M; Joos, S; Schlegelberger, B; Trümper, L H; Feller, A C; Pfreundschuh, M (1995). Identification of genetic imbalances in malignant lymphoma using comparative genomic hybridization. Stem Cells, 13(3):83-87.

[img] PDF - Registered users only
1MB

Abstract

In comparison to leukemias, the clinical relevance of chromosomal aberrations in non-Hodgkin's lymphoma (NHL) is not as well understood. This is primarily due to limitations of chromosomal banding techniques which have been the central methods for cytogenetic analysis. These techniques depend on the availability of fresh tumor tissue and the examination of metaphase cells which may not be representative for the major cell clone in vivo. In contrast, the new technique of comparative genomic hybridization (CGH) allows researchers to obtain a comprehensive view of chromosomal gains and losses by analyzing tumor DNA, which can be prepared from archival tissue samples. Results of CGH studies in three different types of lymphoproliferative disorders are outlined in this paper demonstrating that: (1) in chronic B cell leukemias, chromosomal aberrations are missed by banding analysis in a high proportion of cases, (2) CGH on paraffin-embedded tissue samples can be used for cytogenetic analysis within clinical multicenter trials and (3) DNA amplifications are more frequent in NHL than previously assumed. Thus, it can be expected that CGH will contribute both to the understanding of pathogenetic mechanisms and the identification of clinically relevant chromosome aberrations in NHL.

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Molecular Life Sciences
DDC:570 Life sciences; biology
Language:English
Date:1995
Deposited On:31 Mar 2011 13:02
Last Modified:27 Nov 2013 21:38
Publisher:AlphaMed Press
ISSN:1066-5099
Publisher DOI:10.1002/stem.5530130713
PubMed ID:8747993
Citations:Web of Science®. Times Cited: 9
Google Scholar™
Scopus®. Citation Count: 12

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page