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B lymphocyte-restricted expression of prion protein does not enable prion replication in prion protein knockout mice.


Montrasio, F; Cozzio, A; Flechsig, E; Rossi, D; Klein, M A; Rülicke, T; Raeber, A J; Vosshenrich, C A; Proft, J; Aguzzi, A; Weissmann, C (2001). B lymphocyte-restricted expression of prion protein does not enable prion replication in prion protein knockout mice. Proceedings of the National Academy of Sciences of the United States of America (PNAS), 98(7):4034-4037.

Abstract

Prion replication in spleen and neuroinvasion after i.p. inoculation of mice is impaired in forms of immunodeficiency where mature B lymphocytes are lacking. In spleens of wild-type mice, infectivity is associated with B and T lymphocytes and stroma but not with circulating lymphocytes. We generated transgenic prion protein knockout mice overexpressing prion protein in B lymphocytes and found that they failed to accumulate prions in spleen after i.p. inoculation. We conclude that splenic B lymphocytes are not prion-replication competent and that they acquire prions from other cells, most likely follicular dendritic cells with which they closely associate and whose maturation depends on them.

Prion replication in spleen and neuroinvasion after i.p. inoculation of mice is impaired in forms of immunodeficiency where mature B lymphocytes are lacking. In spleens of wild-type mice, infectivity is associated with B and T lymphocytes and stroma but not with circulating lymphocytes. We generated transgenic prion protein knockout mice overexpressing prion protein in B lymphocytes and found that they failed to accumulate prions in spleen after i.p. inoculation. We conclude that splenic B lymphocytes are not prion-replication competent and that they acquire prions from other cells, most likely follicular dendritic cells with which they closely associate and whose maturation depends on them.

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Additional indexing

Item Type:Journal Article, refereed
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Institute of Neuropathology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:27 March 2001
Deposited On:11 Feb 2008 12:26
Last Modified:05 Apr 2016 12:20
Publisher:National Academy of Sciences
ISSN:0027-8424
Publisher DOI:https://doi.org/10.1073/pnas.051609398
PubMed ID:11274428

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