Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive 

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-19156

Walch, M; Rampini, S K; Stoeckli, I; Latinovic-Golic, S; Dumrese, C; Sundstrom, H; Vogetseder, A; Marino, J; Glauser, D L; Van Den Broek, M; Sander, P; Groscurth, P; Ziegler, U (2009). Involvement of CD252 (CD134L) and IL-2 in the expression of cytotoxic proteins in bacterial- or viral-activated human T cells. Journal of Immunology, 182(12):7569-7579.

[img] PDF - Registered users only
2MB

Abstract

Regulation of cytotoxic effector molecule expression in human CTLs after viral or bacterial activation is poorly understood. By using human autologous dendritic cells (DCs) to prime T lymphocytes, we found perforin only highly up-regulated in virus- (HSV-1, vaccinia virus) but not in intracellular bacteria- (Listeria innocua, Listeria monocytogenes, Mycobacterium tuberculosis, Chlamydophila pneumoniae) activated CTLs. In contrast, larger quantities of IFN-gamma and TNF-alpha were produced in Listeria-stimulated cultures. Granzyme B and granulysin were similarly up-regulated by all tested viruses and intracellular bacteria. DCs infected with HSV-1 showed enhanced surface expression of the costimulatory molecule CD252 (CD134L) compared with Listeria-infected DC and induced enhanced secretion of IL-2. Adding blocking CD134 or neutralizing IL-2 Abs during T cell activation reduced the HSV-dependent up-regulation of perforin. These data indicate a distinct CTL effector function in response to intracellular pathogens triggered via differing endogenous IL-2 production upon costimulation through CD252.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Medical Microbiology
04 Faculty of Medicine > University Hospital Zurich > Clinic and Policlinic for Internal Medicine
DDC:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2009
Deposited On:09 Jun 2009 14:27
Last Modified:27 Nov 2013 23:43
Publisher:American Association of Immunologists
ISSN:0022-1767
Free access at:PubMed ID. An embargo period may apply.
Publisher DOI:10.4049/jimmunol.0800296
PubMed ID:19494280
Citations:Web of Science®. Times Cited: 1
Google Scholar™
Scopus®. Citation Count: 2

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page