Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-19156
Walch, M; Rampini, S K; Stoeckli, I; Latinovic-Golic, S; Dumrese, C; Sundstrom, H; Vogetseder, A; Marino, J; Glauser, D L; Van Den Broek, M; Sander, P; Groscurth, P; Ziegler, U (2009). Involvement of CD252 (CD134L) and IL-2 in the expression of cytotoxic proteins in bacterial- or viral-activated human T cells. Journal of Immunology, 182(12):7569-7579.
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Regulation of cytotoxic effector molecule expression in human CTLs after viral or bacterial activation is poorly understood. By using human autologous dendritic cells (DCs) to prime T lymphocytes, we found perforin only highly up-regulated in virus- (HSV-1, vaccinia virus) but not in intracellular bacteria- (Listeria innocua, Listeria monocytogenes, Mycobacterium tuberculosis, Chlamydophila pneumoniae) activated CTLs. In contrast, larger quantities of IFN-gamma and TNF-alpha were produced in Listeria-stimulated cultures. Granzyme B and granulysin were similarly up-regulated by all tested viruses and intracellular bacteria. DCs infected with HSV-1 showed enhanced surface expression of the costimulatory molecule CD252 (CD134L) compared with Listeria-infected DC and induced enhanced secretion of IL-2. Adding blocking CD134 or neutralizing IL-2 Abs during T cell activation reduced the HSV-dependent up-regulation of perforin. These data indicate a distinct CTL effector function in response to intracellular pathogens triggered via differing endogenous IL-2 production upon costimulation through CD252.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > Institute of Medical Microbiology|
04 Faculty of Medicine > University Hospital Zurich > Clinic and Policlinic for Internal Medicine
|DDC:||570 Life sciences; biology|
610 Medicine & health
|Deposited On:||09 Jun 2009 16:27|
|Last Modified:||23 Nov 2012 17:26|
|Publisher:||American Association of Immunologists|
|Free access at:||PubMed ID. An embargo period may apply.|
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