Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive 

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-1920

Heber, S; Herms, J; Gajic, V; Hainfellner, J A; Aguzzi, A; Rülicke, T; von Kretzschmar, H; von Koch, C; Sisodia, S; Tremml, P; Lipp, H P; Wolfer, D P; Müller, U (2000). Mice with combined gene knock-outs reveal essential and partially redundant functions of amyloid precursor protein family members. Journal of Neuroscience, 20(21):7951-7963.

[img]
Preview
PDF
883Kb

Abstract

The amyloid precursor protein (APP) involved in Alzheimer's disease is a member of a larger gene family including amyloid precursor-like proteins APLP1 and APLP2. We generated and examined the phenotypes of mice lacking individual or all possible combinations of APP family members to assess potential functional redundancies within the gene family. Mice deficient for the nervous system-specific APLP1 protein showed a postnatal growth deficit as the only obvious abnormality. In contrast to this minor phenotype, APLP2(-/-)/APLP1(-/-) and APLP2(-/-)/APP(-/-) mice proved lethal early postnatally. Surprisingly, APLP1(-/-)/APP(-/-) mice were viable, apparently normal, and showed no compensatory upregulation of APLP2 expression. These data indicate redundancy between APLP2 and both other family members and corroborate a key physiological role for APLP2. This view gains further support by the observation that APLP1(-/-)/APP(-/-)/APLP2(+/-) mice display postnatal lethality. In addition, they provide genetic evidence for at least some distinct physiological roles of APP and APLP2 by demonstrating that combinations of single knock-outs with the APLP1 mutation resulted in double mutants of clearly different phenotypes, being either lethal, or viable. None of the lethal double mutants displayed, however, obvious histopathological abnormalities in the brain or any other organ examined. Moreover, cortical neurons from single or combined mutant mice showed unaltered survival rates under basal culture conditions and unaltered susceptibility to glutamate excitotoxicity in vitro.

Item Type:Journal Article, refereed
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Institute of Neuropathology
DDC:570 Life sciences; biology
610 Medicine & health
Language:English
Date:01 November 2000
Deposited On:11 Feb 2008 13:26
Last Modified:27 Nov 2013 17:42
Publisher:Society for Neuroscience
ISSN:0270-6474
Related URLs:http://www.jneurosci.org/cgi/content/full/20/21/7951
PubMed ID:11050115
Citations:Web of Science®. Times Cited: 259
Google Scholar™

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page