Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-1925
Flechsig, E; Shmerling, D; Hegyi, I; Raeber, A J; Fischer, M B; Cozzio, A; von Mering, C; Aguzzi, A; Weissmann, C (2000). Prion protein devoid of the octapeptide repeat region restores susceptibility to scrapie in PrP knockout mice. Neuron, 27(2):399-408.
Mice devoid of PrP are resistant to scrapie and fail to replicate the agent. Introduction of transgenes expressing PrP into such mice restores susceptibility to scrapie. We find that truncated PrP devoid of the five copper binding octarepeats still sustains scrapie infection; however, incubation times are longer and prion titers and protease-resistant PrP are about 30-fold lower than in wild-type mice. Surprisingly, brains of terminally ill animals show no histopathology typical for scrapie. However, in the spinal cord, infectivity, gliosis, and motor neuron loss are as in scrapie-infected wild-type controls. Thus, while the region comprising the octarepeats is not essential for mediating pathogenesis and prion replication, it modulates the extent of these events and of disease presentation.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > University Hospital Zurich > Institute of Neuropathology|
|DDC:||570 Life sciences; biology|
610 Medicine & health
|Date:||01 August 2000|
|Deposited On:||11 Feb 2008 13:26|
|Last Modified:||28 Nov 2013 01:35|
|Citations:||Web of Science®. Times Cited: 188|
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