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Site-Specific Difference 2D-IR Spectroscopy of Bacteriorhodopsin


Andresen, E R; Hamm, P (2009). Site-Specific Difference 2D-IR Spectroscopy of Bacteriorhodopsin. Journal of Physical Chemistry. B, 113(18):6520-6527.

Abstract

We demonstrate the extension of the principle of difference Fourier transform infrared (FTIR) spectroscopy to difference 2D-IR spectroscopy. To this end, we measure difference 2D-IR spectra of the protein bacteriorhodopsin in its early J- and K-intermediates. By comparing with the static 2D-IR spectrum of the protonated Schiff base of all-trans retinal, we demonstrate that the 2D-IR spectrum of the all-trans retinal chromophore in bacteriorhodopsin can be measured with the background from the remainder of the protein completely suppressed. We discuss several models to interpret the detailed line shape of the difference 2D-IR spectrum.

We demonstrate the extension of the principle of difference Fourier transform infrared (FTIR) spectroscopy to difference 2D-IR spectroscopy. To this end, we measure difference 2D-IR spectra of the protein bacteriorhodopsin in its early J- and K-intermediates. By comparing with the static 2D-IR spectrum of the protonated Schiff base of all-trans retinal, we demonstrate that the 2D-IR spectrum of the all-trans retinal chromophore in bacteriorhodopsin can be measured with the background from the remainder of the protein completely suppressed. We discuss several models to interpret the detailed line shape of the difference 2D-IR spectrum.

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11 citations in Web of Science®
12 citations in Scopus®
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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Chemistry
Dewey Decimal Classification:540 Chemistry
Language:English
Date:7 May 2009
Deposited On:21 Oct 2009 08:47
Last Modified:05 Apr 2016 13:17
Publisher:American Chemical Society
ISSN:1520-5207
Funders:Danish Natural Science Research Council, Swiss National Science Foundation
Publisher DOI:10.1021/jp810397u
Permanent URL: http://doi.org/10.5167/uzh-19527

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