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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-19745

Bögli-Stuber, K; Kohler, C; Seitert, G; Glanemann, B; Antognoli, M C; Salman, M D; Wittenbrink, M M; Wittwer, M; Wassenaar, T; Jemmi, T; Bissig-Choisat, B (2005). Detection of Mycobacterium avium subspecies paratuberculosis in Swiss dairy cattle by real-time PCR and culture: a comparison of the two assays. Journal of Applied Microbiology, 99(3):587-97.

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Abstract

AIMS: To compare the two different diagnostic assays for the detection of Mycobacterium avium ssp. paratuberculosis, the aetiological agent of paratuberculosis. METHODS AND RESULTS: Faecal samples were derived from 310 cows, representing 13 commercial dairy herds in various locations in Switzerland with expected increased risk because of a past history of disease. Detection assays for M. avium ssp. paratuberculosis were culture (gold standard) and a newly designed real-time PCR. Real-time PCR identified 31 of 310 animals as positive within this risk population whereas culture identified 20 positive animals. The specificity of real-time PCR was confirmed by DNA sequencing of the PCR product. Depending on the test used, the paratuberculosis prevalence in our tested risk population ranged from 6.5 to 10%. CONCLUSIONS: Real-time PCR and culture data were in good agreement, and real-time PCR generates data in a short time in contrast to culture. SIGNIFICANCE AND IMPACT OF THE STUDY: We consider real-time PCR as a suitable alternative method to culture for the detection of M. avium ssp. paratuberculosis in a national surveillance programme.

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Veterinary Bacteriology
DDC:570 Life sciences; biology
Date:2005
Deposited On:24 Jul 2009 09:38
Last Modified:27 Nov 2013 23:23
Publisher:Wiley-Blackwell
ISSN:1364-5072
Additional Information:This is an electronic version of an Article published in Journal of Applied Microbiology Journal compilation © 2008 The Society for Applied Microbiology
Publisher DOI:10.1111/j.1365-2672.2005.02645.x
PubMed ID:16108801
Citations:Web of Science®. Times Cited: 18
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