Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-19745
Bögli-Stuber, K; Kohler, C; Seitert, G; Glanemann, B; Antognoli, M C; Salman, M D; Wittenbrink, M M; Wittwer, M; Wassenaar, T; Jemmi, T; Bissig-Choisat, B (2005). Detection of Mycobacterium avium subspecies paratuberculosis in Swiss dairy cattle by real-time PCR and culture: a comparison of the two assays. Journal of Applied Microbiology, 99(3):587-97.
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AIMS: To compare the two different diagnostic assays for the detection of Mycobacterium avium ssp. paratuberculosis, the aetiological agent of paratuberculosis. METHODS AND RESULTS: Faecal samples were derived from 310 cows, representing 13 commercial dairy herds in various locations in Switzerland with expected increased risk because of a past history of disease. Detection assays for M. avium ssp. paratuberculosis were culture (gold standard) and a newly designed real-time PCR. Real-time PCR identified 31 of 310 animals as positive within this risk population whereas culture identified 20 positive animals. The specificity of real-time PCR was confirmed by DNA sequencing of the PCR product. Depending on the test used, the paratuberculosis prevalence in our tested risk population ranged from 6.5 to 10%. CONCLUSIONS: Real-time PCR and culture data were in good agreement, and real-time PCR generates data in a short time in contrast to culture. SIGNIFICANCE AND IMPACT OF THE STUDY: We consider real-time PCR as a suitable alternative method to culture for the detection of M. avium ssp. paratuberculosis in a national surveillance programme.
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|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||05 Vetsuisse Faculty > Institute of Veterinary Bacteriology|
|Dewey Decimal Classification:||570 Life sciences; biology|
|Deposited On:||24 Jul 2009 07:38|
|Last Modified:||27 Nov 2013 22:23|
|Additional Information:||This is an electronic version of an Article published in Journal of Applied Microbiology Journal compilation © 2008 The Society for Applied Microbiology|
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